3种致病弧菌感染对大黄鱼血液生化指标的影响  

Effect of three pathogenic Vibrios on the blood biochemical parameters of Pseudosciaena crocea

作　　者：蔡林婷[1] 李思源[1] 葛明峰[1] 胡亚萍[1] 王国良[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,315211

出　　处：《渔业科学进展》2013年第2期65-72,共8页Progress in Fishery Sciences

基　　金：教育部长江学者与创新团队项目(IRT0734);国家公益性行业(农业)科研专项(200903029);浙江省大学生科技创新项目(2010R405038)共同资助

摘　　要：溶藻弧菌Vibrio alginolyticus、哈维弧菌Vibrio harveyi、副溶血弧菌Vibrio parahaemolyticus是引起大黄鱼溃疡病的3种主要致病菌。本研究将试验大黄鱼随机分成4组,分别腹腔注射0.2ml的哈维弧菌、溶藻弧菌、副溶血弧菌和灭菌生理盐水,在感染20d中的不同时间采样,通过检测血清糖类、脂类及蛋白质代谢产物含量,血清蛋白质含量及酶活性,血清电解质含量等有关血液生化指标来比较3种致病弧菌对大黄鱼血液生化指标的影响。结果表明,大黄鱼血液生化指标的变化主要发生在感染后的1～4d,主要表现为总蛋白、白蛋白、球蛋白、谷丙转氨酶、谷草转氨酶、乳酸脱氢酶、K、Fe显著升高(P<0.05),尿素氮、肌酐、血糖、胆固醇、Na、Cl显著降低(P<0.05),甘油三酯、P、Ca、Mg无显著变化(P>0.05),说明致病菌感染对大黄鱼组织器官的生理机能和代谢水平均产生了影响。反映鱼体受感染后从应激反应发生至生化指标变化主要出现在致病菌感染前期,提示血清生化指标的检测可以作为大黄鱼"溃疡病"早期感染的监测指标。Vibrio alginolyticus, V. harveyi and V. parahaemolyticus are main causes of ulcer disease for Pseudosciaena crocea. In this research, we set three experimental treatments of P. crocea which were injected with 0.2ml V. harveyi,V, alginolyticus or V. parahaemo- lyticus, respectively, while injection of normal saline at the same dose was given to the control. To study the impact of the three pathogenic Vibrios on the P. crocea ~s blood biochemical pa- rameters, contents of carbohydrate, lipid, protein, electrolyte and protein metabolism products and enzyme activity in serum were determined and compared by sampling at different time-point for 20d after infection. The results showed that the change of blood biochemical parameters oc- curred mainly in 1-4d after the Vibrio injection. Contents of TP, ALB, GLOB, ALT, AST,LDH, K,and Fe increased significantly（P〈0.05）, and contents of blood urea nitrogen, serum creatinine, blood sugar, cholesterol, Na,and C1 decreased significantly（P〈0.05）, while con- tents of TG, P, Ca, and Mg did not change significantly （P〉0.05）. These observations indica- ted that Vibiro infection has impacts on physiological functions and metabolic levels of P. cro- cea. The study indicates that the stress reaction to the Vibrios occurred mainly during the early stage after infection, and serum biochemical parameters can be used as early-warning indicators of ulcerous infection for P. crocea.

关 键 词：大黄鱼 弧菌 血液生化指标 糖类 脂类 蛋白质 

分 类 号：S941.42[农业科学—水产养殖]