Cd^(2+)对缢蛏谷胱甘肽硫转移酶、磷酸酶活性的影响  

Impact of Cd^(2+) on glutathione S-transferase and phosphatase activity in Sinonovacula constricta

作　　者：陆慧贤[1] 徐永健[1] 

机构地区：[1]宁波大学海洋学院应用海洋生物技术教育部重点实验室,315211

出　　处：《渔业科学进展》2013年第2期119-124,共6页Progress in Fishery Sciences

基　　金：国家科技支撑计划(2009BADB7B02);浙江省自然科学基金项目(Y3110476)共同资助

摘　　要：通过重金属暴露法,研究了不同质量浓度Cd2+(0.005、0.025、0.05、0.1mg/L)在168h内对缢蛏消化腺和鳃组织谷胱甘肽硫转移酶(GST)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性的影响。结果显示,Cd2+对两种组织GST活性均有激活作用,且高浓度比低浓度更易快速诱导其活性,但在暴露的后期(>96h),两个较高浓度组(0.05、0.1mg/L)GST活性均出现下降;两种组织的ACP活性(鳃组织0.005mg/L组除外)在暴露的6h即被显著诱导,并表现出剂效关系,但高浓度组更快达到峰值后就持续下降,于暴露中后期(>72h)被不同程度抑制;消化腺(0.005mg/L浓度组除外)和鳃AKP活性在暴露168h内均表现出"抑制-诱导-抑制"的规律,并且较高浓度组活性反而比低浓度组更易被激活,各组AKP活性被诱导达到最高值后就持续下降直至被再次抑制。Cd2+在低浓度水平下即能引起缢蛏两种组织的GST、ACP、AKP活性变化,表明Cd2+的胁迫可对缢蛏机体的解毒体系和新陈代谢产生影响。Effect of waterborne Cd2＋ at different concentrations （0.005mg/L, 0.025mg/ L, 0.05mg/L, and 0. ling/L） on activities of glutathione S-transferase （GST）, acid phospha- tase（ACP） and alkaline phosphatase （AKP） in digestive glands and gills of Sinonovacula con- stricta were studied. It was found that the GST activity in the two tissues was stimulated by Cd2＋during the exposure time in all treatments, however, those in higher-dose treatments （0.05 mg/L,0.1 mg/L） decreased with the prolonged exposure. The activation of ACP by Cd2＋ in the two tissues began at the 6h time-point, and the activity increased with the increasing concentration of Cd2＋. For ACP activity, the higher-dose group reached the peak value earlierthan the lower-dose group, and was inhibited after 72h. Except 0. 005mg/L dose-treatment in digestive glands, the AKP activities in the two tissues showed a＂decrease-increase-decrease＂ trend during the 168h exposure time in the experiment, and those in higher-dose treatments were stimulated earlier than those in lower ones. AKP activity in each treatment decreased con- stantly after reaching the peak value respectively. The fluctuations in GST, ACP, and AKP ac- tivities in the experiment indicated that exposure to lower concentrations of Cd2＋ could have af- fected the metabolism and detoxification system of the organism.

关 键 词：Cd2+ 缢蛏 谷胱甘肽硫转移酶 磷酸酶 

分 类 号：S968.3[农业科学—水产养殖]