血管紧张素转换酶基因多态性对早发冠心病血瘀证的影响  

Impact of Polymorphism of Angiotensin Converting Enzyme Gene on Premature Coronary Heart Disease with Blood Stasis Syndrome

作　　者：胡志希[1] 胡思远[2] 李琳[1] 李杰[1] 凌智[1] 侯超[1] 

机构地区：[1]湖南中医药大学 [2]湖南师范大学体育学院

出　　处：《中西医结合心脑血管病杂志》2013年第5期515-518,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：湖南省自然科学基金项目(No.11JJ6086);湖南省高校创新平台开放基金重点项目(No.11K049);国家自然科学基金项目(No.81001518;30973717);霍英东教育基金会资助(No.131042)

摘　　要：目的探讨血管紧张素转换酶(ACE)基因多态性对早发冠心病(CHD)血瘀证的影响。方法运用PCR技术对41例早发冠心病(CHD)血瘀证、45例早发冠心病非血瘀证及38名正常对照组的ACE基因型及等位基因频率进行检测,同时检测一氧化氮(NO)和血浆内皮素(ET)含量。结果早发CHD血瘀证DD基因型及D等位基因频率明显高于早发CHD非血瘀证及正常对照组(P<0.05或P<0.01)。早发CHD血瘀证患者ET/NO检测值明显高于早发CHD非血瘀证及正常对照组(P<0.05或P<0.01),且以DD型血瘀证患者最高。结论 ACE基因DD基因型及D等位基因与早发CHD血瘀证发病相关,DD基因型是早发CHD血瘀证的独立危险因素,可增加早发CHD的风险,D等位基因是早发CHD血瘀证的易感基因。早发CHD血瘀证ACE基因多态性与ET/NO密切相关。Objective To discuss the impact of polymorphism of angiotensin converting enzyme （ACE） gene on premature coro- nary heart disease（CHD） with blood stasis syndrome. Methods The ACE genotype and allele frequencies were detected by the poly- merase chain reaction （PCR） in 41 cases with premature coronary heart disease and blood stasis syndrome, 45 cases without blood stasis syndrome and premature CHD,and 38 cases as normal control group. The content of nitric oxide （NO） and plasma endothelin （ET） were detected. Results DD genotype and D allele frequency in cases with premature CHD and blood stasis syndrome were significantly higher than that in cases with premature CHD and without blood stasis syndrome and healthy controls （P〈0.05 or P〈0.01）. ET/NO in patients with premature CHD and blood stasis syndrome were significantly higber than that in patients with premature CHD and without blood stasis syndrome and normal control group （P〈0.05 or P〈0.01）. The highest was patients with DD blood stasis syndrome. Conclusion Pathogenesis of the ACE gene DD genotype and D allele was associated with premature CHD with blood stasis syndrome. DD genotype was an independent risk factor for premature CHD with blood stasis syndrome,it could increase the risk of premature CHD. The D allele was a susceptibility gene for premature CHD with blood stasis syndrome. ACE gene polymorphism was closely related to the ET/NO in premature CHD with blood stasis syndrome.

关 键 词：早发冠心病 血瘀证 血管紧张素转换酶 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病] R256.2[医药卫生—内科学]