OCT4B1在牙髓细胞的表达及其对凋亡相关因子的调控作用  

The expression of OCT4B1 in human dentalp pulp cells and its effect on apoptosis related factors

作　　者：黄蓉[1] 刘路[1] 韦曦[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学研究杂志（电子版）》2013年第2期8-11,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：国家自然科学基金(81070830);广东省科技计划(2010B031600063);广东省医学科研基金(B2012141)

摘　　要：目的研究人牙髓细胞(HDPCs)中八聚体转录结合因子4剪接异构体B1(OCT4B1)的表达及基因转染OCT4B1后对凋亡相关因子的影响。方法实时荧光定量聚合酶链反应(PCR)和荧光原位杂交(FISH)技术检测正常HDPCs中OCT4B1的表达;利用慢病毒载体构建OCT4B1高表达模型并转染HDPCs,检测半胱氨酸天冬氨酸特异蛋白酶(Caspase)3和7的表达变化。结果 OCT4B1表达于正常HDPCs且在第三代中表达最高;上调OCT4B1后与空载体组相比,OCT4B1高表达组Caspase-3、Caspase-7 mRNA表达降低(P<0.05)。结论 HDPCs中上调OCT4B1可抑制Caspase-3和Caspase-7的表达,说明OCT4B1参与细胞应激反应,可能与细胞凋亡相关。Objective This study was to investigate the expression of OCT4B1 in human dental pulp cells （HDPCs） and its effect on apoptosis related factors. Methods The expression of OCT4B1 in normal HDPCs was detected by real-time quantitative PCR and fluorescence in situ hybridization （FISH）. The overexpression model of OCT4B1 was constructed with lentivirus. The expression of Caspase-3 and Caspase-7 was examined after transfection. Results OCT4 splicing variants were expressed in normal HDPCs and the expression peaked at passage 3. Compared with the group transfected with the lentiviral vector, the expression of Caspase-3 and Caspase-7 mRNA of the OCT4B1 overexpression group decreased after the transfection （P 〈 0.05 ）. Conclusions Up-regulation of OCT4B1 in HDPCs caused inhibition of Caspase-3 and Caspase-7. Thus, OCT4B1 may be involved in cellular stress and play a essential role in apoptosis.

关 键 词：牙髓细胞 八聚体转录结合因子4剪接异构体B1 半胱氨酸天冬氨酸特异蛋 白酶3 半胱氨酸天冬氨酸特异蛋白酶7 

分 类 号：R78[医药卫生—口腔医学]