丹参素与川芎嗪对心血管系统的协同作用  

Synergism of tanshinol and tetramethylpyrazine on cardiovascular system in rats

作　　者：黄文东[1] 杨永飞[1] 陈建文[2] 朱邦豪[1] 

机构地区：[1]中山大学中山医学院心脑血管研究中心,广东广州510080 [2]中山大学药学院药理与毒理学实验室,广东广州510089

出　　处：《中国药理学通报》2013年第3期432-436,共5页Chinese Pharmacological Bulletin

摘　　要：目的探讨丹参素与川芎嗪在心血管方面的协同效应,为其复方制剂提供科学依据。方法通过大鼠颈静脉插管滴注受试药(丹参素、川芎嗪、丹参素+川芎嗪),观察对在体大鼠平均动脉压(MAP)、心率(HR)的影响;采用lange-ndorff离体心脏灌流技术对大鼠心脏灌流给予受试药,lab-chart生物信号采集仪器记录观察各受试药对左心室收缩压(LVSP)、左心室发展压(LVDP)、心率(HR)、左心室内压最大上升速率(+dp/dtmax)、左心室内压最大下降速率(-dp/dtmax)以及冠脉流量(CF)等心功能指标的影响。结果在体实验中,丹参素组平均动脉压降低约7%,川芎嗪组降低约12%,而丹参素+川芎嗪组降低约26%,但各组均对大鼠心率影响不明显。在离体心脏灌流实验中,丹参素+川芎嗪组与空白对照组、丹参素组和川芎嗪组相比,具有较强的负性肌力作用,能明显降低LVSP、LVDP、+dp/dtmax和HR,且升高-dp/dtmax和CF,其差异具有统计学意义(P<0.05)。结论丹参素、川芎嗪联合应用对心血管的效应有协同作用。Aim To investigate the synergism on Cardiovascular system between tanshinol （TSN） and tetramethylpyrazine（TMP） from traditional Chinese medicine and provide a scientific basis for their compound preparation. Methods Through neck intravenous administration （TSN, TMP and TSN ＋ TMP）, their effects on the mean artery pressure （MAP） and the heart rate （HR） of rats were observed in vivo. Meanwhile, the cardiovascular effects of TSN, TMP and TSN ＋ TMP were investigated using the isolated Langendorff perfused heart of rats. Myocardial hemodynamic parameters were monitored and recorded, such as left ventricular systolic pressure （ LVSP）, left ventrieular developed pressure （ LVDP ） , heart rate （ HR ） , the maximum change rate of left ventricular pressure rise and fall（ ± dp/dtmax ） and coronary flow（CF）. Results TSN could reduce MAP by 7%, TMP by 12% and TSN ＋ TMP by 26%. But HR was not obviously affected. In the langendorff experiment, it was found that compared with the control group, TSN group and TMP group, the TSN ＋ TMP group had a stronger negative inotropic action which could remarkably reduce LVSP, LVDP, ＋ dp/dtmax and HR, while increase -dp/dtmax and CF（P 〈 0. 05）. Conclusion The combination of the two shows synergistic or additive effect, which ＇may be related to the antagonism of calcium ion channel.

关 键 词：丹参素 川芎嗪 复方制剂 LANGENDORFF 心血管系统 协同作用 

分 类 号：R-332[医药卫生] R282.71