应用不同代次兔肾细胞培养风疹病毒松叶株的研究  

Study on matsuba strain of rubella virus cultured in rabbit kidney cells of different passages

作　　者：刘晓凡[1] 邢家强[1] 魏至栋[1] 窦强[1] 林杰[1] 徐小明[1] 

机构地区：[1]兰州生物制品研究所有限责任公司,甘肃省疫苗工程技术研究中心,兰州730046

出　　处：《微生物学免疫学进展》2013年第2期7-10,共4页Progress In Microbiology and Immunology

摘　　要：目的为提高兔肾细胞产量用于增加风疹病毒生产。方法 SPF级家兔肾细胞经传代培养10代,对不同代次兔肾细胞进行遗传稳定性、外源因子及兔脑原虫检测后接种风疹病毒松叶株(Matsuba strain)的试验。结果兔肾细胞产量得到提高,由1对兔肾平均生产1瓶细胞增加为8瓶,细胞核型检查传至第10代的细胞染色体数目与初代细胞一致,细胞培养物均一性提高。第0～第3代细胞病毒培养物滴度间无显著性差异,χ2检验返回相关性的P值均大于0.95。结论第1～第3代细胞培养风疹病毒与第0代相比,可获得相同滴度的病毒培养物,p3代兔肾细胞应用于疫苗生产可显著提高细胞及病毒产量。Objective It is on the purpose to increase yield for cultured rabbit kidney cells for enhancement of rubella virus production. Methods The primary rabbit kidney cells in SPF level were subcultured for 10 passages. The live matsuba strain of rubella virus was inoculated to all of the diffent passaged cells, respectively , which were subjected to tests such as karyotype analysis, examination of eneephalitozoon cuniculi and factors of exterenal sources. Results The homogeneity for cultured rabbit kidney cells was increased. The results of the karyotype analysis showed that the chromosomes of subcul-tured cells are consistent with these of primary cells. It has no notable difference for virus titers propagated on 0 to 3 passa- ges. The P of the X^2 test is more than 0.95 （ P〉0. 05 ）. Conclusion The virus titers on 1 to 3 passages are same with those on 0 passage. The 3 passage cell is a good substrate for culture of matsuba strain.

关 键 词：风疹减毒活疫苗(兔肾细胞) 病毒滴度 

分 类 号：R392.1[医药卫生—免疫学]