青光安有效组份对兔眼滤过术后滤过道瘢痕组织成纤维细胞和Ⅰ型胶原蛋白的影响  

Effects of effective components of Qing Guang An on inoblast and typeⅠcollagen of scarring tissue of filtration canal after glaucoma surgery

作　　者：刘艳[1] 彭清华[1] 

机构地区：[1]湖南中医药大学第一附属医院眼科,中国湖南省长沙市410007

出　　处：《国际眼科杂志》2013年第5期845-849,共5页International Eye Science

基　　金：国家自然科学基金资助项目(No.81273807);教育部博士点基金专项课题(No.20124323110006);国家中医药管理局中医眼科学重点学科建设项目;中国湖南省自然科学基金重点资助项目(No.11JJ2050);中国湖南省教育厅科研基金重点资助项目(No.06A052;No.10A094);中国湖南省卫生厅科研基金项目(No.204084);国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目~~

摘　　要：目的:观察青光安4种有效组份对青光眼术后滤过道瘢痕组织成纤维细胞和Ⅰ型胶原蛋白的影响,探讨青光安有效组份抗青光眼术后滤过道瘢痕化的效果及作用机制。方法:将青光安有效组份作用于滤过性手术后兔眼(D,E,F,G组),通过与空白组(A组)、模型组(B组)、MMC组(C组)的比较,观察青光安4种有效组份对青光眼术后滤过道瘢痕组织成纤维细胞和Ⅰ型胶原蛋白的影响。结果:术后MMC组及有效组份2组(E组)眼压回升缓慢,第4wk时眼压仍为最小,该两组眼压值与其他组眼压值比较差异均有统计学意义(P<0.05)。成纤维细胞个数组间比较,除组份1组与组份3组间比较,差异无统计学意义(P>0.05)外,其他各组间比较差异均有统计学意义(P<0.05)。Ⅰ型胶原蛋白的表达除C组与E组、B组与G组、F组与G组间胶原表达差异无统计学意义(P>0.05),其他组间两两比较结果,差异具有统计学意义(P<0.05)。结论:青光眼术后滤过道瘢痕化,是导致术后眼压异常回升、滤过性手术失败的重要原因。青光安有效组份2和MMC都可通过抑制成纤维细胞增殖和Ⅰ型胶原蛋白表达明显减少瘢痕组织增生,具有明显的抗青光眼术后滤过道瘢痕化的作用。AIM ： To observe effects of four effective components ot Qing Guang An on inoblast and type I collagen of scarring tissue of filtration canal after glaucoma surgery, and discuss their effect and function mechanism on preventing its formation. METHODS： Four effective components of Qing Guang An were used in rabbit eyes after glaucoma surgery （Group D. E. F. G）, with the blank group CA）, model group （B）, MMC group comparison （C. Effects of four effective components of Qing Guang An on inoblast and type I collagen of scarring tissue of filtration canal after glaucoma surgery were observed. RESULTS： After surgery, MMC group （C）, effective component 2 （E） groups of intraocular pressure rose slowly. At the fourth week, intraocular pressure remained minimum. The compare of these two groups of intraocular pressure and those of group A, B, D, F, G had significance on statistics. Comparing with the number of inoblast of each group, except group D and E, which result was P〉 0.05 and had no obvious significance on statistics, others were significant. Comparing with the expression of type I collagen, except group C and E, B and G, F and G, result of others had obvious significance on statistics showing as P〈0.05. CONCLUSION： The scaring of filter canal after glaucoma surgery is main cause of the filtration surgery＇s failure assuming abnormal backup intraocular pressure. The effective componment 2 of Qing Guang An and MMC can obviously inhibit the multiplication of inoblast and the expression of type I collagen, reduce the hyperplasia of scar tissue, which has obvious effect for preventing scarrina of filtration canal.

关 键 词：青光眼 滤过道瘢痕化 青光安有效组份 成纤维细胞 Ⅰ型胶原蛋白 

分 类 号：R775[医药卫生—眼科]