HIF-1α及VEGF在增生性糖尿病视网膜病变视网膜前膜中的表达  

The expression of HIT-1α and VEGF in proliferative diabetic retinopathy epiretinal membranes

作　　者：曹建峰[1] 庞东渤[1] 叶文婕 

机构地区：[1]辽宁医学院第一附属医院眼科,中国辽宁省锦州市121001 [2]扬州中医院心内科,中国江苏省扬州市225000

出　　处：《国际眼科杂志》2013年第5期857-860,共4页International Eye Science

摘　　要：目的:研究缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)及其靶基因血管内皮生长因子(vascular endothelial growth factor,VEGF)在增殖型性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)视网膜前膜的表达,探讨其在PDR发生发展中的作用。方法:针对26例PDRⅤ～Ⅵ期患者,行玻璃体切除+剥膜术(有合并视网膜脱离者加行视网膜复位术),术中取出视网膜前膜行免疫组织化学染色,观察视网膜前膜中HIT-1α与VEGF和血管内皮标志物CD34的表达。同时行术前血糖、甘油三脂、糖化血红蛋白、是否合并视网脱进行分类并行多因素Logistic回归分析。结果:PDRⅤ～Ⅵ期视网膜前膜血管内皮细胞中,表达HIT-1α者24例(92.3%),VEGF者15例(57.7%),血管内皮标志物CD34者26例(100%)。血管内皮标志物CD34分别与HIT-1α(r=0.556,P=0.028)和VEGF(r=0.745,P=0.001)直线相关。术前血糖、甘油三脂、糖化血红蛋白、是否合并视网脱是PDR视网膜前膜中表达因子HIT-1α,VEGF和CD34表达的相关因素。结论:HIT-1α和VEGF在糖尿病视网膜病变视网膜前膜中明显表达。HIT-1α和VEGF可能在PDR的发病中起重要作用。To investigate the expression of the hypoxia - inducible factor-In （HIF-la） and the protein products of its target gene vascular endothelial growth factor （VEGF） in proliferative diabetic retinopathy （PDR） epiretinal membranes. METHODS： Totally 26 patients who have been obtained epiretinal membranes （PDR V-VI） were performed pars- pinna vitrectomy. They were removed the epiretinal membrane intraoperation and to observe HIF-la, VEGF and expression of vascular endothelial marker CD34 in epiretinal membranes through immunohistochemical method. RESULTS：Vascular endothelial cells of PDR expressed HIF-la in 24 cases （92.3%） and VEGF in 15 cases （57.7%） in membranes, respectively. There were significant correlations between the numbers of blood vessels expressing CD34 and HIF-I （r= 0. 556, P= 0. 028）. There were significant correlations between the numbers of blood vessels expressing CD34 and VEGF（r=0.745,P=0.001）. CONCLUSION= HIF- la and VEGF may play an important role in the pathogenesis of PDR.

关 键 词：增生性糖尿病视网膜病变 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R774.1[医药卫生—眼科] R587.1[医药卫生—临床医学]