复方甘草酸苷对TNBS结肠炎大鼠肠道炎症的影响  

Effect of Compound Glycyrrhizin on Intestinal Inflammation in Rats with TNBS-induced Experimental Colitis

作　　者：徐进[1] 杜勇[1] 卢光新[1] 杨艳果[1] 张少君[1] 周春芳[1] 吕飞[1] 

机构地区：[1]湖北医药学院附属人民医院消化内科,442000

出　　处：《胃肠病学》2013年第4期221-224,共4页Chinese Journal of Gastroenterology

基　　金：2011年度湖北省教育厅科学技术研究项目(B20112120)

摘　　要：背景:复方甘草酸苷的主要成分甘草酸具有抗炎和免疫调节作用。临床上复方甘草酸苷用于溃疡性结肠炎的治疗取得一定疗效。目的:观察复方甘草酸苷对实验性结肠炎大鼠肠道炎症的影响及其可能机制。方法:40只大鼠随机分为四组,三组以TNBS/乙醇诱导结肠炎,并分别予0.9%NaCl(模型对照组)、复方甘草酸苷和SASP灌肠14 d,正常对照组仅以0.9%NaCl灌肠14 d。实验期间评估疾病活动指数(DAI)。治疗结束后处死大鼠,行结肠黏膜大体和组织学损伤评分,免疫组化法检测结肠组织NF-κB p65、iNOS表达,ELISA法检测血清IL-8、IL-4水平。结果:模型对照组大鼠DAI、结肠黏膜大体和组织学损伤评分以及结肠组织NF-κB p65、iNOS表达和血清IL-8水平显著高于正常对照组(P<0.05),血清IL4水平显著低于正常对照组(P<0.05)。复方甘草酸苷组大鼠上述指标均较模型对照组显著好转(P<0.05),与SASP组相比无明显差异。结论:复方甘草酸苷对结肠炎的治疗作用可能与抑制NF-κB活化,进而下调iNOS以及促炎细胞因子如IL-8表达,上调抗炎细胞因子如IL4表达有关。Glycyrrhizic acid, the main component of compound glycyrrhizin, is demonstrated to have anti- inflammatory and immunoregulatory properties. Recently, compound glycyrrhizin has been used in the treatment of ulcerative colitis with some efficacy in clinical practice. Aims： To study the effect and possible mechanism of compound glycyrrhizin on intestinal inflammation in rats with experimental colitis. Methods： Forty rats were randomly divided into four groups. In three groups, experimental colitis was induced by TNBS/ethanol, and then the rats received intrarectal treatment with 0.9% NaC1 （ model control group）, compound glycyrrhizin and SASP, respectively, for 14 days. Another 10 rats served as normal controls received intrarectal 0.9% NaC1 only for 14 days. Disease activity index （ DAI ） was assessed during the experiment. After treatment, all rats were sacrificed for evaluation of macroscopic and histological damage of the colon, lmmunohistochemistry was used to detect the expressions of NF-KB p65 and iNOS in colonic mucosa, and the serum levels of IL-8 and IL-4 were measured by ELISA method. Results： DAI, macroscopic damage score, histological damage score, and expressions of NF-KB p65 and iNOS in colonic mucosa, as well as the serum level of IL-8 were significantly higher, and the serum level of IL-4 was significantly lower in model control group than in normal control group （ P 〈 0.05 ）. In compound glycyrrhizin-treated group, all parameters mentioned above were significantly improved in comparison with model control group （ P 〈 0.05 ）. No significant differences were found between glycyrrhizin-treated group and SASP-treated group. Conclusions： Compound glycyrrhizin might exert therapeutic effect on colitis by suppressing NF- KB p65 activation, which leads to down-regulation of iNOS and proinflammatory cytokines （ e. g. IL-8 ） and up-regulation of anti-inflammatory cytokines （ e.g. IL-4）.

关 键 词：复方甘草酸苷 结肠炎 NF-κB 诱生型一氧化氮合酶 白细胞介素8 白细胞介素4 

分 类 号：R574.62[医药卫生—消化系统]