乳腺癌患者BRCA-1蛋白表达与HER-2基因扩增的相关性  

Relationship between Protein Expression of Breast Cancer Susceptibility Gene 1 and Gene Amplification of Human Epidermal Growth Factor Receptor 2 among Patients with Breast Cancer

作　　者：利基林 唐艳萍[1] 梁新强[1] 欧超[1] 邓伟[1] 沈筱芸[1] 容敏华[1] 骆成飘[1] 张力图[1] 

机构地区：[1]广西医科大学附属肿瘤医院实验研究部,南宁市530021

出　　处：《广西医学》2013年第4期396-398,共3页Guangxi Medical Journal

基　　金：广西科学研究与技术开发计划项目(1298003-2-8)

摘　　要：目的探讨乳腺癌组织乳腺癌易感基因1(BRCA-1)蛋白表达与原癌基因人类表皮生长因子受体2(HER-2)的基因扩增关系。方法分别采用免疫组化法和荧光原位杂交法检测84例乳腺癌(导管内癌32例,浸润性导管癌36例,纤维腺瘤16例)组织中的BRCA-1蛋白表达和HER-2基因扩增情况。结果 BRCA-1蛋白阳性表达63例,阳性表达率为75.0%(63/84)。BRCA-1蛋白表达与淋巴结转移有关(P<0.05)。与不同组织类型无关(P>0.05)。HER-2基因扩增阳性40例,HER-2基因扩增阴性44例。36例有淋巴结转移患者HER-2扩增阳性率为86.1%(31/36),明显高于48例无淋巴结转移患者的27.1%(13/48)(P<0.01)。HER-2基因扩增阳性组BRCA-1蛋白阳性表达率为90.0%(36/40),高于HER-2基因扩增阴性组的22.7%(10/44)(P<0.01)。结论 BRCA-1蛋白表达、HER-2基因扩增与乳腺癌的发生发展有关,BRCA-1蛋白表达与HER-2基因扩增可能存在相关性。Objective To investigate the relationship between protein expression of breast cancer susceptibility gene 1（BRCA-1） and gene amplification of human epidermal growth factor receptor 2（HER-2） from surgical specimens of patients with breast cancer .Methods Immunohistochemistry and chromogenic in situ hybridization were used to detect protein expressions of BRCA-1 and gene amplification of HER-2 in 84 tumor tissues from breast cancer patients including 32 cases of intraductal carcinoma ,36 cases of infiltrating ductal carcinoma ,and 16 cases of fibroadenoma, respectively.Results The positive expression of BRCA-1 protein was found in 63 cases,the positive rate was 75.0%（63/84）.The protein expressions of BRCA-1 in the different tumor histology type groups showed no significant difference （P〉0.05）,but in different lymph node metastasis were significantly different （P〈0.05）.Forty cases showed positive HER-2 gene amplification and 44 cases showed negative HER-2 gene amplification.The positive rate（86.1%,31/36） of HER-2 gene amplification in the breast cancer patients with lymph node metastasis was higher than that in the patients （27.1%,13/48） without lymph node metastasis（P〈0.01）.The positive rate of BRCA-1 protein（90.0%,36/40） in the group with HER-2 gene amplification was higher than that（22.7%,10/44） in the group without HER-2 gene amplification （P〈0.01）.Conclusion The protein expression of BRCA-1 and HER-2 gene amplification correlate with the development and progression of breast cancer.The protein expression of BRCA-1 may be related to HER-2 gene amplification.

关 键 词：乳腺癌 BRCA-1 HER-2 

分 类 号：R737.9[医药卫生—肿瘤]