红花注射液对溃疡性结肠炎大鼠MUC2,IL-6表达的影响  

Effects of Injection of Carthamus Tinctorius on Expression of MUC2 and IL-6 in Rats Model with Ulcerative Colitis

作　　者：王鹤鸣[1] 原皓[1] 赵雪曼[1] 董文华[1] 韩雪[1] 

机构地区：[1]潍坊医学院内科学教研室,山东潍坊261053

出　　处：《潍坊医学院学报》2013年第2期129-132,共4页Acta Academiae Medicinae Weifang

摘　　要：目的通过观察红花注射液(ICT)对2,4,6-三硝基苯磺酸(2,4,6-TNBS)诱导的大鼠溃疡性结肠炎(UC)的疗效及其对结肠黏膜中粘蛋白(MUC2)、白细胞介素6(IL-6)表达的影响,探讨其作用机制。方法将30只健康雄性Wistar大鼠随机分为对照组10只、模型组10只与红花组10只。采用TNBS灌肠法复制大鼠UC模型,红花组同时给予ICT干预治疗,模型组与对照组给予等量生理盐水。造模10d后,对大鼠行疾病活动指数(DAI)、结肠大体形态评分及组织学损伤评分,分别用免疫组织化学法及实时荧光定量PCR(RT-PCR)技术观察大鼠结肠黏膜中MUC2,IL-6的表达。结果与对照组相比,实验组与模型组DAI评分、结肠大体形态评分、组织学损伤评分均升高,而实验组比模型组显著改善(P<0.01);与对照组相比,模型组MUC2表达显著降低,而红花组又比模型组显著升高(P<0.05)。模型组IL-6表达显著升高,而红花组又比模型组显著降低(P<0.05)。结论 ICT对UC大鼠疗效显著,其作用机制之一可能是通过上调结肠黏膜MUC2及下调IL-6的表达,增强肠黏膜屏障作用.减轻类症反应。Objective To investigate the effects of injection of carthamus tinctorius（ICT） on the expression of mucin 2（MUC2） and interleukin-6（ IL-6 ） iu the colonic mucosa of rats with ulcerative colitis and to study the function and mechanism of SS in rats with ulcer- ative colitis. Methods Thirty Wistar rats were divided randomly into control group（ 10）, ICT group（10） and TNBS group（10）. Ulcerative colitis was induced in the ICT group and TNBS group by rectal administration of trinitrobenzene sulphonic acid（TNBS）. AI the same time ICT group was treated with ICT. Control group and model group were treated with sodium chloride. After ten days,all rats were killed. Disease ac- tivity index（DAI） ,mucosal lesion area and histological damage scores were assessed,and the expression of MUC2 and IL-6 in the colonic mueosa was detected by immunohistochemistry and real-time fluorescent quantification polymerase chain reaction. Results Compared with the model group,the DAl,mucosal lesion area and histological damage scores in ICT group were significantly decreased（ P 〈 0.05）; Com- pared with model group,the levels of MUC2 in ICT gruup were significantly increased（ P 〈 0.05） ;the levels of IL-6 in experimental group were significantly decreased（ P 〈 O. 05 ）. Conclusion ICT can exert protective effects against rat ulcerative colitis perhaps by upregulating MUC2 expression and downregulating IL-6 expression.

关 键 词：结肠炎 渍疡性 红花注射液 MUC2 IL-6 

分 类 号：R574.62[医药卫生—消化系统]