5-氟尿嘧啶对人SGC-7901胃癌细胞系增殖及p53β,p53,Bax表达的影响  

The Effects of 5-fluorouracil on the Proliferation and Expression of p53β,p53 and Bax in Human SGC-7901 Gastric Cancer Cell Lines

作　　者：曹玉[1] 贾飞飞[1] 季万胜[2] 

机构地区：[1]潍坊医学院内科学教研室,山东潍坊261053 [2]潍坊医学院附属医院消化内科

出　　处：《潍坊医学院学报》2013年第2期147-149,共3页Acta Academiae Medicinae Weifang

基　　金：山东省优秀中青年科学家科研奖励基金资助课题(项目编号:BS2010SW034)

摘　　要：目的观察5-氟尿嘧啶(5-FU)作用于p53突变型SGC-7901胃癌细胞系后,抑制细胞增殖及p53β,p53,Bax表达的变化,探讨其发生机制。方法不同浓度5-FU作用于SGC-7901胃癌细胞系,采用MTT法检测细胞的抑制率;巢式逆转录多聚酶链反应(nRT-PCR)检测p53β,p53,Bax在mRNA水平上表达的变化。结果MTT结果显示,5-FU对SGC-7901胃癌细胞系的增殖具有抑制作用,且呈剂量和时间双效应,不同浓度5-FU(0mg/L,25mg/L,50mg/L,100mg/L)作用于SGC-7901胃癌细胞系24,48,72h后,其抑制率逐渐增高(F=36.643,66.030,50.769,均P<0.01);RT-PCR结果显示,SGC-7901胃癌细胞系中p53β,p53,Bax在mRNA水平上的表达变化不明显(F=3.581,0.669,2.945,均P>0.05)。结论 5-FU作用于突变型p53.胃癌细胞系后,共抑制细胞增殖可能与p53突变的具体位点有关,亦可能与p53非依赖性途径的信号传递有关。Objective To study the effecls of 5-flurouracil（5-FU）on cell inhibition of proliferation amt the expression of p5313, p53 and Bax in mutant p53 SGC-7901 gastric cancer cell lines,and to investigate its mechanism. Methods SGC-7901 gastric cancer cells were interfered with different concentrations of 5-FU, and MTT assay was applied to detect the growth inhibition rates, p5313, p53 and Bax mR- NA was detected by nested reverse lrans（wiption-polymerase chain reaction（nRT-PCR）.Results In MTF assay,it was shown that the SGC- 7901 gastric cancer cell lines were inhibited hy 5-FU. The growth inhibition rate was positively related to the concentration and culturing time. SGC-7901 gastric cancer cell lines were treated by different concentrations of 5-FU （0mg/L,25mg/L,50mg/L, 100mg/L） for 24,48 and 72 hours respectively, and the inhibition rate increased gradually （ F = 36. 643,66. 030,50. 769, P 〈 0.01 respectively）. With RT-PCR analy- sis, the mRNA levels of f）53 β, p53 amt Bax were m）t changed significantly with the growing 5-FU concentra!ions （ F = 3.581,0. 669,2. 945, P 〉 0.05 respectively）. Conclusion The inhibition effects of cell proliferation of mutant p53 gastric cancer cell lines treated by 5-FU may be associated with p53 mutation sites or its signal of the p53-independent pathway.

关 键 词：5-FU 胃癌细胞系 p53β P53 Bax 

分 类 号：R735.2[医药卫生—肿瘤]