p53、14-3-3σ、miR-365在UVB致HaCaT细胞G_2/M期阻滞中的作用  

The roles of p53,14-3-3σ and miR-365 in the G_2/M phase arrest induced by UVB irradiation of HaCaT cells

作　　者：郭院霞[1] 王颖慧[1] 李明芳 刘明[1] 周美娟[1] 

机构地区：[1]南方医科大学在读医学硕士研究生,广州市510505

出　　处：《实用医学杂志》2013年第9期1388-1391,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81202152,81172634);广东省博士启动基金(编号:S2011040003686);教育部高等学校博士点专项科研基金(编号:20094433110004)

摘　　要：目的:研究30mJ/cm2的UVB照射后,HaCaT细胞周期的改变及其可能机制。方法:以人永生化上皮细胞(HaCaT)为研究对象,波长305nm的UVB为干预因子,首先观察了HaCaT细胞在30mJ/cm2的UVB作用后其细胞周期的变化;其次观察了UVB照后不同时间点p53蛋白、14-3-3σ蛋白表达的改变;最后用miR-365高表达的HaCaT细胞分析了miR-365在UVB所致了的周期阻滞中的可能作用。结果:HaCaT细胞在30mJ/cm2的UVB作用后18h出现较明显的G2/M期阻滞;p53、磷酸化的p53以及14-3-3σ蛋白在UVB照射后均有明显升高;miR-365高表达的HaCaT细胞,14-3-3σ蛋白的mRNA表达下降,且在UVB照射后无明显改变。结论:30mJ/cm2的UVB照射可诱导HaCaT细胞发生G2/M期阻滞,p53、14-3-3σ、miR-365可能在其中发挥了重要作用。Objective To study the changes of cell cycle of HaCaT cells induced by 30 mJ /cm2 UVB irradiation and its possible mechanism.Methods The cell cycle of human epithelial cells（HaCaT）was first analyzed after irradiated by 30 mJ /cm2 of UVB（305 nm）;then the expressions of p53,phosphorylated p53 and 14-3-3σ protein at different time points,and the role of miR-365 in cell cycle arrest induced by UVB irradiation of miR-365 high-expressing HaCaT cells were observed.Results Obvious G2 /M phase arrest was observed in HaCaT cells at 18 h after 30 mJ /cm2 of UVB irradiation.The expressions of p53,phosphorylated p53 and 14-3-3σ protein were significantly increased after UVB irradiation.The expression of 14-3-3σ mRNA was decreased in miR-365 high-expressing HaCaT cells,and had no obvious change after UVB irradiation.Conclusion G2 /M phase arrest of HaCaT cells could be induced by 30 mJ /cm2 UVB irradiation,and p53,14-3-3σ and miR-365 may play important roles in this process.

关 键 词：中波段紫外辐射 G2 M期阻滞 P53 14-3-3σ miR-365 

分 类 号：R363[医药卫生—病理学]