上海汉族人群MICA基因第5外显子微卫星多态性研究  

Study on polymorphism of MICA exon 5 microsatellite in Shanghai Han population *

作　　者：郭忠慧[1] 许玲娣[2] 范丽安[2] 葛瑜[2] 姚芳娟[2] 杨珏琴[2] 

机构地区：[1]上海市血液中心 [2]上海第二医科大学上海市免疫学研究所,200025

出　　处：《中华医学遗传学杂志》2000年第5期332-335,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金!(39970 742);卫生部科研基金!(98-2-30 8);上海-联合利华研究基金! (9911)

摘　　要：目的　了解上海地区汉族人群 MICA基因第 5外显子微卫星多态性分布 ,以及 MICA基因与其紧密连锁的 HL A- B基因位点的关系。方法　用 PCR-异源双链分析法 ,对 175名正常无关个体的MICA基因第 5外显子微卫星多态性的分布进行研究。结果　 (1)上海地区汉族人群 MICA基因第 5外显子存在 5种等位基因 ,其中 MICA* A5最为常见 (39.14% ) ,其次为 * A5 .1(2 2 .2 9% ) ;(2 ) MICA等位基因与 HL A- B位点之间存在一定的连锁不平衡 ,如 :MICA* A5与 HL A- B15 ,MICA* A4与 HL A- B2 7等。结论　 MICA基因第 5外显子多态性分布虽没有 HL A经典 类、 类基因那样明显的人种和地区差异 。Objective To investigate the polymorphism distribution of MICA gene exon 5 microsatellite in Shanghai Han population and study the relationship of MICA gene and its closely linked gene HLA B. Methods The microsatellite polymorphism of MICA exon 5 in 175 unrelated healthy individuals were investigated using PCR heteroduplex analysis. Results (1)There are 5 alleles of the microsatellite polymorphism in exon 5 of the MICA gene in Shanghai Han population, the gene frequency of MICA*A5 is the highest(39.14%), the next is MICA*A5.1(22.29%). (2)There is strong linkage disequilibrium between the HLA B and MICA STR loci, e.g. MICA*A5 and HLA B15, MICA*A4 and HLA B27.Conclusion The polymorphism distribution of MICA gene and the linkage disequilibrium between MICA and HLA B have deviations in different ethnic groups.

关 键 词：MICA基因 人类白细胞抗原 微卫星多态性 

分 类 号：R394[医药卫生—医学遗传学]