青心酮对兔动脉粥样硬化血管细胞黏附因子-1蛋白表达的影响  

The effect of DHAP on the expression of VCAM-1 in atherosclerotic rabbits

作　　者：王琳[1] 刘同美[1] 张代娟[1] 刘江月[1] 

机构地区：[1]潍坊医学院病理生理教研室,山东潍坊261053

出　　处：《济宁医学院学报》2013年第2期100-102,105,共4页Journal of Jining Medical University

摘　　要：目的探讨新西兰大白兔动脉粥样硬化病变中血管细胞黏附因子-1(VCAM-1)的表达和青心酮抗动脉粥样硬化作用。方法 18只新西兰大耳白兔随机分为常规饲料组、胆固醇饲料组和青心酮组,饲养12周。实验前后检测血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL),采用免疫组化和Western blot法测定主动脉VCAM-1阳性表达百分比和蛋白的表达。结果青心酮显著降低TC、TG和LDL水平(P<0.05)。胆固醇组和青心酮组病变局部VCAM-1的阳性细胞百分比和蛋白的表达含量明显高于常规饲料组(P<0.05)。但青心酮组动脉粥样硬化改变较胆固醇组明显减轻,VCAM-1阳性百分比和蛋白的表达量较胆固醇饲料组明显降低(P<0.05)。结论动脉粥样硬化的发生伴有血管细胞黏附因子的高度表达,青心酮不但有调脂作用,且具有抑制黏附分子表达和抗动脉粥样硬化的作用。Objective To explore the effect of DHAP on the expression of VCAM-1 in atherosclerotic rabbits. Methods 18 rabbits were randomly divided into three groups： normal group,cholesterol diet group,DHAP treat- ment group. All rabbits were fed for 12 weeks. Serum TC, TG, LDL were measured in 0 and 12 weeks in three groups. At 12 week, we detected the aortic VCAM-1 positive percentage by immunohistochemistry assay and the expression of VCAM-1 protein by Western blot.Results DHAP can obviously decrease the level of TC TG and LDL （P〈~0.05）. The measurement of VCAM-1 positive percentage and the expression of VCAM-1 protein both in cho- lesterol diet group and DHAP treatment group were higher than normal group（P^0.05）, but pathologic changes in DHAP treatment group were lower than cholesterol group（P^0.05）. The measurement of VCAM-1 positive per- centage and the expression of VCAM-1 protein were also lower than cholesterol diet group（P^0.05 ）. Conclusion VCAM-1 can be highly expressed in atherosclerosis. DHAP can not only decrease cholesterol but also inhibit ex- pression of VCAM-1 which has the effect of anti-atherosclerosis.

关 键 词：青心酮 血管细胞黏附因子-1 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]