HBV复制及其基因分型与HBV相关慢性肝病关系的临床研究  

Correlation between Hepatitis B Virus(HBV) Replication/HBV Genotype and HBV-Related Chronic Liver Disease

作　　者：江必武[1] 叶丽华[2] 王平[1] 

机构地区：[1]武汉市第一医院消化内科,武汉430022 [2]华中科技大学同济医学院附属同济医院感染科,武汉430030

出　　处：《华中科技大学学报（医学版）》2013年第2期231-233,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(No.2011CDB302);武汉市科技指导计划项目(No.Z200960123009);武汉市卫生局临床医学科研资助项目(No.WX12A06)

摘　　要：目的探讨乙型肝炎病毒(HBV)复制情况及其基因分型与HBV相关慢性肝病病变程度之间的关系。方法对500例HBV相关慢性肝病患者进行乙肝标记物、HBV-DNA定量检测,采用PCR微板核酸杂交-ELISA法检测HBV基因型,观察HBV-DNA基因型分布,同时观察慢性乙型肝炎、肝炎后肝硬化、肝细胞癌中基因型的分布差异。结果基因型以B型和C型为主,分别为47.1%和52.9%。慢性肝炎中以B型为主,肝硬化中以C型占优,而在肝细胞癌中B型和C型所占百分比差异无统计学意义。结论 C型HBV感染与乙肝病情加重、肝硬化发生有一定关系;而B型HBV感染者在慢性肝炎中多见,提示预后相对较好。Objective To examine the correlation between hepatitis B virus（HBV） replication/HBV genotype and HBV- related chronic liver disease. Methods HBV markers and the quantity of HBV-DNA were detected in 500 patients with chronic HBV infections. The HBV genotypes of all the patients were determined by PCR microplate hybridization-ELISA method. The difference in the HBV genotype among chronic HBV disease,liver cirrhosis and hepatocellular carcinoma was evaluated. Results HBV genotypes B and C were commonly seen in patients suffering HBV-related chronic liver disease,and the positive rate of these two genotypes reached 47.1 % and 52.9 % respectively. Genotype B was the most dominant type in chronic hepatitis and genotype C in liver cirrhosis. The percentage of genotype B was not significantly different from that of genotype C in hepatoeel- lular carcinoma. Conclusion Genotype C is associated with the exacerbation of hepatitis and liver cirrhosis. Genotype B infection is more common in chronic hepatitis and it suggests a relatively good prognosis.

关 键 词：慢性肝病 乙型肝炎病毒 基因型 

分 类 号：R575.1[医药卫生—消化系统]