γδT细胞对人宫颈癌HeLa细胞增殖和凋亡的影响  

Effect of γδT Cells on the Proliferation and Apoptosis of Human Cervical Cancer HeLa Cells

作　　者：李华[1] 韦立蓓[1] 王育斌[2] 

机构地区：[1]武汉科技大学附属天佑医院妇产科,湖北武汉430064 [2]武汉科技大学医学院生理学系

出　　处：《湖北科技学院学报（医学版）》2013年第1期15-17,共3页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：武汉科技大学人才启动项目(编号530033);武汉科技大学青年科技骨干培养计划项目(编号2013xz030)

摘　　要：目的探讨γδT细胞对人宫颈癌HeLa细胞增殖和凋亡的影响。方法从宫颈癌患者手术切除的肿瘤组织分离肿瘤浸润淋巴细胞,固相抗体包被法体外扩增得到γδT细胞(效应细胞),与人宫颈癌HeLa细胞(靶细胞)按照不同的比例共培养24h或48h后,MTT法检测HeLa细胞增殖情况,流式细胞术(FCM)检测细胞的凋亡。结果γδT细胞与HeLa细胞按不同效靶比共培养24h和48h后,MTT法检测结果显示,增殖率均较对照组显著降低(P<0.05)并显示了剂量依赖性,效靶比为32∶1时,50%以上的细胞停止增殖;γδT细胞与HeLa细胞(效靶比为4∶1)共培养24h后FCM分析结果显示,HeLa细胞凋亡率较对照组显著增强(P<0.01)。结论γδT细胞对人宫颈癌HeLa细胞增殖有抑制作用并呈现剂量依赖性,对细胞凋亡有促进作用。Objective To investigate the effect of γδT cells on the proliferation and apoptosis of human cervical cancer HeLa cells. Methods γδT ceils from TILs （ tumor infiltrating lymphocytes） of the human cervi- cal cancer specimens were expanded using the solid-phase antibody coated method. Expanded γδT cells （ effec- tor cells） co-cultured with HeLa ceils （target cells） in different ratios for 24h or 48h ,then HeLa cells prolifera- tion was measured by MTr assay and apoptosis was measured by flowcytometry （FCM）. Results When expand- ed γδT cells as effector cells（E） co-cultured with HeLa cells that were set as target cells（T） in different E/T ratios for 24h or 48h,the MTT results showed γδT cells markedly inhibited cell proliferation of HeLa cells in dose dependent manners when compared with the control group（ P 〈0.05 ）. More than 50% of HeLa cells stops proliferating at E/T ratios of 32：1 ;the results of FCM showed apoptosis rate of HeLa cells was significantly in- creased at E/T ratios of 4：1 for 24h when compared with the control group（ P 〈 0.01 ）. Conclusion γδT cells could inhibit the proliferation of human cervical cancer HeLa ceils in dose dependent manners and induce apop- tosis.

关 键 词：ΓΔT细胞 宫颈癌 HELA细胞 细胞增殖 细胞凋亡 

分 类 号：R730.23[医药卫生—肿瘤]