HPLC法同时测定氨咖黄敏胶囊中三种成分  

Simultaneous determination of paracetamol,caffeine and chlorphenamine maleate in Ankahuangmin capsule by HPLC

作　　者：孙超[1] 崔潇[1] 成秉辰[1] 何志一[1] 

机构地区：[1]哈尔滨市食品药品检验检测中心,哈尔滨150525

出　　处：《哈尔滨商业大学学报（自然科学版）》2013年第2期145-147,175,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

摘　　要：建立同时测定氨咖黄敏胶囊中对乙酰氨基酚、咖啡因、马来酸氯苯那敏含量的HPLC法.色谱柱为Agilent ZORBAX SB-C18(150 mm×4.6 mm,5μm);流动相为磷酸二氢铵溶液(取0.1 mol/L磷酸二氢铵溶液1 000 mL,加磷酸1 mL,混匀)-乙腈(82∶18),流速为1.0 mL/min,检测波长为216nm,柱温27℃.线性范围为对乙酰氨基酚2.020～25.245μg,r=0.999 6;咖啡因0.099～1.240μg,r=1.000 0;马来酸氯苯那敏0.020～0.256μg,r=1.000 0;平均回收率:对乙酰氨基酚97.1%,RSD=0.78%;咖啡因99.2%,RSD=0.70%;马来酸氯苯那敏98.6%,RSD=0.89%.本方法简便、快速、准确,可更好地控制氨咖黄敏胶囊的质量.This paper established a method for the determination of paracetamol, caffeine and chlorphenamine maleate in Ankahuangmin capsule by HPLC. The analytical column was Ag- ilent ZORBAX SB -C18（ 150 mm ×4.6 mm,5 μm） , with the column temperature of 27℃. The mobile phase consist of ammonium biphosphate- acetonitrile was 82： 18, with flow rate of 1.0 mL/min. The detection wavelength was at 216 nm. The linear ranges of paracetamol, caffeine and chlorphenamine maleate were at 2. 020 ~ 25. 245 μg （ r = 0. 999 6 ） ,0.099 ~ 1. 240 μg （ r = 1. 000 0）, 0.020 - 0.256 p,g （ r = 1. 000 0）, respectively. The average recover- ies of paracetamol, caffeine and chlorphena -mine maleate were 97.1% （RSD = 0.78% ）, 99.2% （RSD = 0.70% ） ,98.6% （RSD = 0.89% ）, respectively. The method was simple, accurate,it could be used for the quality control of Ankahuangrmin capsule.

关 键 词：高效液相色谱法 氨咖黄敏胶囊 对乙酰氨基酚 咖啡因 马来酸氯苯那敏 

分 类 号：R284[医药卫生—中药学]