新疆维吾尔族妇女宫颈病变中DAPK及PTEN表达的研究  

The Expression of DAPK and PTEN in cervical lesions in Uyghur Women in Xinjiang

作　　者：刘晓婉[1] 昆多孜.乌赛那洪 刘兆春[2] 张玉毅 马玉兰 

机构地区：[1]新疆维吾尔自治区人民医院妇科,新疆乌鲁木齐830001 [2]石河子大学医学院第一附属医院妇产科,新疆石河子832000 [3]湖北省襄阳市第一人民医院妇产科,湖北襄阳441100

出　　处：《新疆医学》2013年第2期26-30,共5页Xinjiang Medical Journal

摘　　要：目的:探索DAPK及PTEN与维吾尔族女性宫颈癌及癌前病变的相关性。方法:取维族妇女正常及炎症的宫颈组织30例、宫颈上皮内瘤变Ⅰ级病变组织30例、宫颈上皮内瘤变Ⅱ/Ⅲ级病变组织30例、宫颈鳞状细胞癌组织30例,采用免疫组化检测DAPK及PTEN基因的在蛋白水平的表达。结果:DAPK及PTEN基因分别在蛋白水平的表达百分比在非癌组及鳞组中分别为93.3%、83.3%、60.0%、33.3;83.3%、73.3%、56.7%、23.3%,两者均显示其在宫颈癌组阳性百分表达率明显减少,与非癌组差异有显著性(P<0.05)。结论:新疆维吾尔族妇女宫颈病变组织中DAPK及PTEN蛋白水平表达减少,其与新疆维族妇女宫颈病变呈负相关关系:两者可作为宫颈癌前病变的预警信号及宫颈癌的预后评估。Objective： To investigated the expression and significance of DAPK and PTEN in normal cervix, and chronic cervicitis,cervical intraepithelial neoplasia Ⅰ （CIN Ⅰ ）, CIN Ⅱ/Ⅲ and invasive squamous carcinoma of cervix from Uygur females in Xinjiang.Methods： 30 cases with normal cervix and chronic cervicitis, 30 cases with CIN Ⅰ ,30 cases with CIN Ⅱ/Ⅲ and 30 cases of cervical squamous cell carcinoma were tested for expression of DAPK and PTEN by immunohistochemistry SP method. Results： The positive rates of DAPK and PTEN protein in normal cervix and chronic cervicitis, cervical intraepithelial neoplasia Ⅰ （CIN Ⅰ ）,CIN Ⅱ/Ⅲ and invasive squamous carcinoma of cervix were 93.3%,83.3%,60.0%,33.3% ;83.3 %,73.3%,56.7%,23.3%,respectively. Both DAPK and PTEN expression in the SCC group was significantly lower than that in the other groups （P〈0.05）. Conclusion： The expression of DAPK and PTEN protein in cervical carcinoma is low, it was negative correlated with Uygur females in cervical lesions in Xinjiang, The detection of DAPK and PTEN expression can be used as early warning signs of cervical precancerous lesions and evaluation of prognosis for cervical cancer prognosis.

关 键 词：宫颈鳞癌 DAPK PTEN 免疫组化 维族妇女 

分 类 号：R739.41[医药卫生—肿瘤]