盐度驯化对褐牙鲆幼鱼渗透压调节和能量代谢的影响  

Effects of Salinity Acclimatization on the Osmoregulation and Energy Metabolism of Brown Flounder(Paralichthys olivaceus)

作　　者：黄国强[1,2] 张灵燕[2] 李洁[2] 唐夏[2] 

机构地区：[1]广西海洋研究所广西海洋生物技术重点实验室,广西北海536000 [2]中国海洋大学水产学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2013年第5期44-51,共8页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30600462)资助

摘　　要：将体重(20±1.8)g的褐牙鲆(Paralichthys olivaceus)幼鱼以不同的盐度日变幅由盐度33驯化至盐度5和47。结果表明,褐牙鲆幼鱼血清渗透压与驯化到的盐度均呈明显的正相关性。以日变幅3.5驯化至5和47的过程中,鳃丝Na+-K+-ATP酶活力均呈升高趋势,以其他变幅驯化则鳃丝Na+-K+-ATP酶活力无显著变化。以日变幅7驯化,褐牙鲆幼鱼血清渗透压达到稳定的时间均为2d。以日变幅14,驯化至5后,达到稳定的时间为4d,驯化至47,实验期间内未达到稳定。以不同盐度日变幅驯化至5和47后,在之后的适应期间,不同处理Na+-K+-ATP酶活力的变动趋势均不同。实验中未发现驯化过程中及驯化到高盐度和低盐度后适应阶段褐牙鲆幼鱼的耗氧率与鳃丝Na+-K+-ATP酶活力有明显相关性。褐牙鲆幼鱼的氧氮比在盐度驯化过程中均出现下降和波动,在适应阶段均未恢复至对照水平。研究结果为丰富褐牙鲆适应环境盐度的机制提供了资料,可为增养殖中进行盐度驯化提供参考。Juvenile brown flounder （Paralichthys olivaceus） with an average body weight of 20± 1.8 g living in salinity 33 were acclimated to as high as saninity 47 and as low as salinity 5 in this study. The plasma osmolality of the juvenile are positively related to the final salinity. An ascending trend of activity of Na^＋-K^＋-ATP ase appeared when the juvenile fish were acclimated to either salinity 47 or sanility 5 at a changing rate of 3.5/d. However, the change of activity of Na^＋-K^＋-ATPase was not significant when the juvenile fish were acclimated at salinity change rates of 14/d and 7/d. The plasma osmolality of juvenile fish acclimated to 47 and 5 at a salinity change rate of 7/d reached a stead level in 2d. A period of 4 days was needed for juvenile fish to be acclimated to 5 at a salinity change rate of 14/d. However, the plasma osmolality of juvenile fish acclimated to 47 at a salinity change rate of 14/d did not reach a steady level in the experimental period. The activity of Na^＋-K^＋-ATPase fluctuated and showed different trends when the fish were acclimated at different daily salinity change rates. No direct relation between oxygen con- sumption rate and activity of Na^＋-K^＋-ATPase was detected. The atomic ratio of oxygen to nitrogen （N. O） declined and fluctuated in the salinity acclimatization and did not recovered to the control level at the end. The results of present experiment provided information for exploring the mechanism of adaptation to environmental salinity in P. olivaceus, and reference to the salinity acclimatization in culture and enhancement.

关 键 词：褐牙鲆 盐度驯化 渗透压调节 NA+-K+-ATP酶 能量代谢 

分 类 号：S917[农业科学—水产科学]