NS398对人胃癌裸鼠移植瘤生长的影响及其机制研究  

Effects of NS398 on the Growth of Gastric Cancer Xenografts and Its Mechanism

作　　者：林德安[1] 李佳[1] 慕光川[1] 胡武杰[1] 刘志明[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,广西南宁530021

出　　处：《医学信息》2013年第8期364-366,共3页Journal of Medical Information

摘　　要：目的观察环氧化酶-2抑制剂NS398对胃癌SGC-7901细胞裸鼠皮下移植瘤生长的影响，并探讨其机制。方法建立胃癌裸鼠皮下移植瘤模型，成瘤后随机分为实验组和对照组（n=8），实验组给予NS3983mg／kg腹腔注射，3次／w，共5w。对照组给予等量DMSO。绘制肿瘤生长曲线，测定移植瘤体积及重量并计算抑瘤率．免疫组织化学染色技术和实时荧光定量PCR（Real-Timepcv．）法检测移植瘤COX-2、survivin和bcl-2的表迭情况，TUNEL法检测肿瘤细胞凋亡。结果相比对熙纽，实验组肿瘤生长速度明显减缓，凋亡指数明显增加[（187±0．75）％比（6．38±2．1）蜘，抑瘤率为59％。实验组COX-2、survivin和bd-2蛋白和mRNA表达均明显下降（P〈005、．结论NS398可有效抑制胃癌裸鼠皮下移植癌的生长．并诱导肿瘤细胞的凋亡．其机制可能与抑制COX-2、survivin和bcl-2的表达有关。Objective To evaluate the effects of NS398,a cyclooxygenase-2 inhibitor ,on the growth of subcutaneous tumor xenograft of SGC-7901 gastric cancer cells and its mechanism. Methods The xenograft model of human gastric carcinoma with SGC-7901 gastric cancer cells was reproduced in nude mice.The nude mice were randomly divided into two groups, control group and experimental group （8 eaeh）.The mice were consecutively given intraperitoneal injection administration with 3mg/kg NS398 in experimental group,ar DMSO in control group for 5 weed.The tumor sizes were measured for tumor curve.Determination of the volume and quality of the transplanted tumor and the tumor growth inhition was calculated.TUNEL assay was used for apoptosis evaluation. The expressions of protein and mRNA of COX-2, survivin and bel-2 in transplanted tumor were detected by immunohistoehemistry （IHC） and Real-Time PCR.Results Compared to the control group,the tumor growth decreased significantly, and apoptosis index increased significantly [（ 18.7~0.75 ）% ~＇（6.38~2.1 ）%] in experimental groups. The inhibition rate of tumor weight were 59. IHC and Real-Time PCR analyses showed that NS398 effectively inhibited the expression of protein and mRNA of COX-2,survivin and bcl-2 ,compared with that in the control group （P〈0.05）. Conclusion NS398 can inhibit the growth of gastric cancer xcnograft in nude mice, and induce apoptosis of tumor cells,the mechanism may be related to the inhibition of cox-2, survivin and bcl-2 expression.

关 键 词：环氧化酶-2 生存素 B细胞淋巴瘤 白血病-2 胃肿瘤 NS398 

分 类 号：R739.87[医药卫生—肿瘤]