HMGBl与白血病细胞分化的关系及其作用的实验研究  

Experimental Study on the Relationship of HMGB1 Gene with the Differentiation and Role of Leukemia Cells

作　　者：张文[1] 宋冠华[1] 张之勇[1] 韦若颍[2] 潘素飞[1] 史露露[1] 毕可红[2] 姜国胜[1] 

机构地区：[1]山东省医学科学院基础医学研究所和济南大学(山东省医学科学院医学与生命科学学院),山东济南260062 [2]山东省千佛山医院血液科,山东济南250014

出　　处：《医学信息》2013年第8期393-394,共2页Journal of Medical Information

摘　　要：目的探讨高迁移率族蛋白I（HMGBl）在白血病细胞分化中的作用。方法通过RT—PcR和Westernblot方法检测急性早幼粒细胞白血病细胞（HL-60）经全反式雏甲酸（ATRA）24h、48h、72h、96h诱导后HMGB1在mRNA水平和蛋白水平的表达情况。结果HL-60在经全反式维甲酸诱导向粒系分化的同时．HMGBl在mRNA水平和蛋白水平的表连均随时间的延长呈降低的趋势。结论与在实体瘤中相比，HMGB1在白血病中同样高表达．且其表达程度与白血病细胞的分化程度呈女相关。Objective To discuss the role of the high mobility group protein 1 （HMGB1） in leukemia＇s differentiation.Methods Through the RT-PCR and Western blot method to detect the expression of HMGB1 mRNA level and protein level in acute promyelocytic leukemia cell （HL-60） induced by all-trans-retinoic acid from 24h to 96h.Results HMGB1 in the mRNA levels and protein levels were decreased expression in HL-60 when it was induced differentiation into granulocytes by all-trans retinoie acid. Conclusion Compared with solid tumors, HMGB1 was overexpression in leukemia and negatively related to the maturity degree of leukemia cells.

关 键 词：高迁移率族蛋白 白血病 全反式维甲酸 

分 类 号：R733.71[医药卫生—肿瘤]