注射用地龙冻干粉针对大鼠颈动脉血栓形成的影响  

Anti-thrombotic Effect of Lyophilized Earthworm Powder for Injection

作　　者：梁超[1] 刘冬洁[2] 高瑞辰[3] 韩丽敏[3] 曲轶[3] 沈鑫[3] 朱久新[3] 侯云龙[3] 乔国芬[3] 

机构地区：[1]哈尔滨医科大学附属第一医院 [2]大庆油田总医院肿瘤科 [3]哈尔滨医科大学药理教研室

出　　处：《神经药理学报》2011年第5期14-18,共5页Acta Neuropharmacologica

基　　金：黑龙江省教育厅科学技术研究项目(No.L12511320);哈尔滨医科大学大学生创新创业训练计划

摘　　要：目的:评价注射用地龙冻干粉针的抗血栓作用。方法:SD雄性大鼠40只,利用血栓生成仪复制颈动脉血栓模型,模型成功后72 h,将动物随机分成空白组、地龙冻干粉针1.8,0.9 mg.kg-1剂量组及阳性药疏血通注射液对照组,每组10只,各组大鼠分别尾静脉注射(iv)生理盐水、地龙冻干粉针1.8,0.9 mg.kg-1剂量溶液和疏血通注射液(0.1 mL.kg-1)12 d后,剥离颈动脉血栓,测定血栓重量,并对各组大鼠腹主动脉取血,利用ELISA法测定血浆组织型纤溶酶原激活剂(tissue type plasminogen activator,t-PA)含量,采用观察法测定各组大鼠的凝血时间(clotting time,CT)、血浆复钙时间(recalcification time,RT),利用血小板聚集仪测定血小板最大聚集率(maximal platelet aggregation rate,PAgR-max)。结果:与空白组相比较,地龙冻干粉针1.8 mg.kg-1剂量组大鼠血栓重量显著减少(P<0.05);阳性药疏血通组与地龙冻干粉针0.9 mg.kg-1剂量组大鼠血浆t-PA含量显著升高(P<0.05);阳性药疏血通组、地龙冻干粉针1.8 mg.kg-1剂量组大鼠CT、RT显著延长(P<0.05),0.9 mg.kg-1剂量组RT显著延长(P<0.05),且地龙冻干粉针对RT延长呈剂量依赖性。结论:注射用地龙冻干粉针具有显著的溶解血栓的作用,并能够抑制内源性凝血途径,溶血栓作用可能与提高血浆t-PA含量有关。Objective：To observe the anti-thrombotic effects of lyophilized earthworm powder for injection （LEPI）. Methods：Rats were randomly divided into four groups：blank control,low dose ELPI, high dose ELPI and Shuxuetong injection group. Carotid artery thrombus was induced by a thrombus formation instrument. Seventy-two hours after thrombus induction, rats were treated with saline （2 mL·kg^-1 ）,low dose of LEPI （0.9 mg·kg^-1 ）,high dose of LEPI （1.8mg·kg^-1）, and Shuxuetong solution （0.1 mL·kg^-1 ） , respectively, daily for 12 consecutive days. Blood was sampled for plasma t-PA level determination using ELISA method,CT and RT values were measured in all groups,and the maximal platelet aggregation rate （PAgt^-max） was tested using LBY-NJ4 method. Results：Compared with control group,thrombus weight in high dose LEPI group was decreased significantly （P〈0.05 ）; plasma t-PA levels in both low dose and positive control groups were significantly elevated （P〈0.05）; CT and RT in Shuxuetong treated control group and high-dose LEPI group were significantly prolonged （P〈0.05）;RT was dose- dependently prolonged by LEPI treatment. Conelusion：LEPI possesses significant thrombolytic effects, possibly by inhibiting intrinsic coagulation pathway through increasing plasma level of t-PA.

关 键 词：地龙冻干粉针 颈动脉血栓形成 抗血栓 

分 类 号：R743.32[医药卫生—神经病学与精神病学] R965[医药卫生—临床医学]