miR-133a mimics对骨肉瘤细胞系MG63增殖和凋亡作用的影响  

Effect of miR-133a Mimics on the Proliferation and Apoptosis of Osteosarcoma MG63 Cells

作　　者：李丽华[1] 李梅[1] 赵华福[1] 王丽[2] 邓英虎[1] 张余[1] 

机构地区：[1]广州军区广州总医院骨科医院,广东广州510010 [2]广州军区广州总医院医学实验科,广东广州510010

出　　处：《现代生物医学进展》2013年第10期1869-1872,共4页Progress in Modern Biomedicine

基　　金：全军医学科研"十二五"重点项目(bws11c065);穗科信字[2011]233-32号;全军医学科研"十二五"面上项目(cws11c268)

摘　　要：目的:研究采用miR-133a mimics瞬时转染骨肉瘤细胞系MG63对其细胞增殖和凋亡作用的影响。方法:采用miR-133a mim-ics瞬时转染骨肉瘤细胞系MG63,以miR-negative contro(lNC)mimics作为阴性对照。通过RT-PCR法检测miR-133a在转录水平的表达,CCK法检测其对增殖的影响,采用流式细胞仪检测miR-133a mimics对MG63细胞凋亡作用的影响。利用生物信息学方法预测miR-133a的靶基因,并对其靶基因进行基因功能分析。结果:(1)miR-133a mimics成功转染MG63细胞,并经RT-PCR检测可有效表达。(2)转染48h后,miR-133a mimics组与miR-NC mimics组比较,增值活性明显降低(P<0.01)。(3)miR-133amimics组与miR-NC mimics组和正常细胞相比,其凋亡率显著上升(P<0.01)。(4)生物信息学方法预测miR-133a的靶基因,部分发挥抑制细胞增殖和凋亡的作用。结论:miR-133a对人骨肉瘤细胞MG63的增殖和凋亡能力可能存在调控作用,可能成为骨肉瘤治疗的潜在候选靶点。Objective： The osteosarcoma MG63 cells which are tranfected with miR-133a mimics were studied on the effect of proliferation and apoptosis.Methods： Human osteosarcoma MG63 cells were transfected with miR-133a mimics and miR-negative control（NC） mimics by lipofectamine 2000.The expression of miR-133a in MG63 was verified by RT-PCR.The proliferation and apoptosis of MG63 were tested by CCK and flow cytometer,respectively.The target genes of miR-133a were forecasted by bioinformatics tools,and the function of miR-133a were analyzed.Results： The miR-133a mimics were transfected into MG63 cells successfully,and RT-PCR results indicated that it was effectively expressed.MG63 cells tranfected with miR-133a mimics revealed remarkable change in growth compared to the miR-NC mimics（P0.01） after 48 h.The miR-133a mimics group showed significant apoptosis in MG63（P0.01）.The possible target genes of miR-133a were forecasted by bioinformatics tools,and part of the target genes played a biological function of inhibiting cell proliferation and pro-apoptosis.Conclusion： miR-133a may regulate the proliferation and apoptosis of osteosarcoma cells,and may be potential candidate target for the osteosarcoma treatment.

关 键 词：miR-133a MIMICS MG63细胞 细胞增殖 细胞凋亡 

分 类 号：R738[医药卫生—肿瘤]