长链非编码RNA H19促进肝癌细胞增殖和侵袭  

LncRNA H19 Promotes Hepatocellular Carcinoma Cell Proliferation and Invasion

作　　者：熊伟民[1] 李明峰[1] 宋佳鸿 岳海燕[1] 席素娟 李朝燕[1] 

机构地区：[1]解放军第455医院消化肿瘤科,上海200052

出　　处：《现代生物医学进展》2013年第10期1945-1948,共4页Progress in Modern Biomedicine

基　　金：南京军区医药卫生科研项目(08MA035)

摘　　要：目的:肝癌是严重危害人类健康的一类恶性肿瘤,但其发病机制目前仍不明确。虽然长链非编码RNA的异常表达与多种肿瘤的发生密切相关,但在肝癌中的报导尚不多见。因此,本文将探讨长链非编码RNA H19在肝癌组织和正常组织中表达差异,进一步检测其对肝癌细胞增殖和侵袭活性的调控及其分子机制,为开发长链非编码RNA的临床诊断试剂提供实验依据。方法:收集20例肝癌手术患者的癌变组织和20例正常组织,通过real time PCR检测H19的表达差异。在转染或干扰掉该H19后,采用MTT的方法检测HepG2细胞的增殖活性,通过Transwell小室的方法检测HepG2细胞的侵袭活性。结果:real time PCR检测发现H19 mRNA在肝癌组织中高表达,而在正常组织中低表达。过表达H19导致HepG2细胞过度增殖,敲除该lncRNA,细胞增殖和侵袭活性被抑制。结论:H19在肝癌组织中的表达明显高于正常肝组织,并且过表达H19导致肝癌细胞增殖活性增加,而敲除H19会导致肝癌细胞的增殖和侵袭活性明显减弱。该LncRNA在肝癌组织中的过量表达有助于肝癌的临床诊断,同时H19在肝癌的恶性肿瘤生物活性中发挥着重要的作用,因此,H19是一个潜在的治疗肝癌的药物作用靶点,为开发新的抗肿瘤药物提供了新的治疗靶点。Objective： To investigate the expression profile of LncRNA H19 in hepatocellular carcinoma samples,the final goal of this study is to clarify the role of H19 in hepatocellular carcinoma cells’ proliferation and invasion.Methods： Real time PCR was used to determine the expression of H19.MTT assay and Transwell assay were performed to investigate overexpression or depletion of H19 on HepG2 cells’ proliferation and invasion.Result： LncRNA H19 overexpressed in hepatocellular carcinoma samples.Moreover,overexpression of H19 enhanced HepG2 cell proliferation,however,knocking down H19 resulted in reduce of proliferation and invasion.Conclusion： H19 plays an important role in regulations of hepatocellular carcinoma’s proliferation and invasion.The elevated H19 expression in hepatocellular carcinoma tissue will facilitate clinical diagnosis and imply that this is a potential target for hepathocellular carcinoma therapy.

关 键 词：H19 长链非编码RNA 肝癌 HEPG2细胞 增殖 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]