周期性张应力对面颌肌细胞Na^+/K^+-ATPase功能活性影响  

Effects of Na^+/K^+-ATPase Activity by Cyclic Tensile Stretch in Cultured Maxillofacial Skeletal Muscle Cells in Vitro

作　　者：贾萍萍[1] 杨竹丽 袁晓 郑如松[3] 薛敏[1] 孙仙蕊[1] 刘丽娟[1] 姚如永[4] 

机构地区：[1]青岛大学医学院附属青岛市立医院,山东青岛266021 [2]青岛市立医院口腔科,山东青岛266071 [3]青岛大学医学院附属医院海阳分院,山东海阳265100 [4]青岛大学医学院附属医院中心实验室,山东青岛266021

出　　处：《现代生物医学进展》2013年第13期2456-2459,2467,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31170891)

摘　　要：目的:本研究在成功构建面颌肌细胞体外培养一力学刺激模型的基础上,探讨机械张应力对细胞内Na+水平和面颌肌细胞Na+/K+-ATPase功能活性的影响。方法:本实验采用Blua法,对SD大鼠乳鼠面颌肌细胞进行体外原代培养、鉴定并绘制生长曲线;取第3代细胞接种于细胞加力板上,采用Forcel四点弯曲加力装置,对细胞分别施加1 h、2 h、4 h、8 h、12 h、16 h、24 h和48 h的张应力刺激,后测定细胞内Na+水平变化和Na+/K+-ATPase功能活性改变。结果:(1)Na+水平变化:与对照(不加力)组相比,加力1h细胞内Na+显著增加(P<0.05),并随加力时间延长逐渐降低,加力至12h时降到正常水平,再延长加力时间Na+无明显变化。(2)Na+/K+-ATPase功能活性变化:分别施加不同时间的周期性张应力后,Na泵的活性在加力8小时后才开始增加(0.5725mmolPi/mgPr.hr),随加力时间延长进一步增加,48小时后达到最大值(0.8963mmolPi/mgPr.h)。结论:周期性张应力刺激会引起细胞内Na+的改变,并可以增加骨骼肌细胞Na+/K+-ATPase的活性。Objective： To investigate the effect of mechanical strain on Na＋/K＋-ATPase activity after a SD rats maxillofacial skeletal muscle cell culture model was built.Methods： Firstly,Skeletal muscle cultures were prepared from maxillofacial skeletal muscle from 2 to 3 days neonatal SD rats as described by Blau＇s method.The cyclic tensile stress was exposed to the third passage maxillofacial skeletal muscle Cells for 1,2,4,8,12,16,24 and 48 hours,respectively,using a ＂Forcel＂ four point bending device.After mechanical loading,Na＋/K＋-ATPase activities were determined to investigated the effect of strain on Na＋/K＋-ATPase activities.Results：（1） Intracellular Na＋： Compared with the control group,Na＋ was elevated at the beginning of stretch,but return to control levels when the treatment continued after stretched for 12 hours.（2） Na＋/K＋-ATPase Activity： Na＋/K＋-ATPase activity was increased above the baseline activity after stretch for 8 hours,and significantly increased at 48 hours.Conclusion： The cyclic tensile stretch stimuli can affect the intracellular Na＋,and increase Na＋/K＋-ATPase activity in cultured Maxillofacial Skeletal Muscle Cells.

关 键 词：面颌肌细胞 功能矫形 Na+ K+-ATPase 张应力刺激 

分 类 号：Q953[生物学—动物学] R783.5[医药卫生—口腔医学]