酶法提取虎杖中白藜芦醇的工艺优化  

Optimization of Extraction Process of Resveratrol from Polygoni cuspidati by Enzymatic Method

作　　者：王林[1] 何宗琴[1] 郑在[1] 尹若春[1] 

机构地区：[1]安徽大学生命科学学院,安徽合肥230601

出　　处：《安徽农业科学》2013年第8期3339-3340,3381,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(60908022;51205400);亚热带农业生物资源保护与利用国家重点实验室开放课题(KSL-CUSAb-2012-03);大学生创新创业训练计划项目(cxcy2012049)

摘　　要：[目的]对酶法提取虎杖中白藜芦醇的工艺条件进行优化。[方法]采用单因素试验与正交试验相结合的方法,确定用酶法从虎杖中提取白藜芦醇的最佳工艺条件。[结果]从虎杖中提取白藜芦醇的最佳工艺为:料水比1∶15(V/V),漆酶含量0.30%,纤维素酶含量0.50%,木聚糖酶含量0.40%,β-葡聚糖酶含量0.20%,pH 4.4,在此条件下白藜芦醇的得率达0.592%。[结论]该研究为白藜芦醇的开发及综合利用提供了研究基础。[Objective] The paper was to optimize the extraction process of resveratrol from Polygoni cuspidati by enzymatic method.[Method] The best conditions of extraction of resveratrol from P.cuspidati by enzymatic method were determined by using the method of single factor experiment combined with orthogonal experiment.[Result] The best extraction process of resveratrol from P.cuspidati was that,the ratio of material and water was 1∶15(V/V);laccase content was 0.30%;cellulose content was 0.50%;xylanase content was 0.40%;β-glucanase content was 0.20%;pH 4.4,and the yield of resveratrol under these conditions was up to 0.592%.[Conclusion] The study provided a research basis for the development and utilization of resveratrol.

关 键 词：虎杖 白藜芦醇 酶 

分 类 号：S567[农业科学—中草药栽培]