缺失C8-K3片段重组天坛痘苗病毒的免疫策略研究  

Optimization of immunization strategies of recombinant vaccinia virus Tiantan strain with C8-K3 region deletion

作　　者：刘铮[1] 刘颖[1] 王书晖[1] 张其程[1] 王荣敏[1] 侯爵[1] 刘畅[1] 吉栩[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心,性病艾滋病预防控制中心,北京102206

出　　处：《中国热带医学》2013年第3期263-266,共4页China Tropical Medicine

基　　金：国家科技重大专项(No.2012ZX10001-008);国家自然科学基金国际合作项目(No.81020108030);传染病重点实验室归国人员启动经费(No.2011SKLID303);中国疾控青年基金(No.2012A105)

摘　　要：目的探索不同的DNA疫苗初免-重组痘苗病毒加强免疫策略的效果,为建立最佳免疫方案提供依据。方法对小鼠进行DNA疫苗初免,之后分别加强免疫一针、两针和三针重组痘苗病毒VTT△C8-K3-gag,在不同时间点采取血样及制备脾细胞,检测针对痘苗病毒和HIV特异性体液免疫及细胞免疫应答。结果三种免疫策略中,DNA疫苗初免-VTT△C8-K3-gag加强免疫两次的效果最佳,诱导的针对p55的结合抗体滴度达到106,分泌IFN-γ的T细胞数为342/106细胞。针对痘苗病毒的体液和细胞免疫应答显著低于加强免疫三针诱导的免疫反应。结论DNA疫苗初免-VTT△C8-K3-gag加强免疫两针可诱导较高水平的HIV免疫应答,同时保证了较低水平的载体免疫反应。Objective To optimize the DNA prime-recombinant VTT boost regimen,we compared three immunization strategies in BALB/C mice.DNA vaccine priming followed by VTT△C8-K3-gag boosting twice elicited stronger humoral and cellular responses against HIV gag than that induced by VTT△C8-K3-gag boosting once.The p55 specific binding antibody titer was 106,and IFN-γ＋ T cells reached 342/million cells when stimulated by Gag peptide.Meanwhile,strategy of VTT △C8-K3-gag boosting twice induced significant lower vaccinia specific antibody and cellular responses than that elicited by VTT△C8-K3-gag boosting three times.These data suggest that DNA vaccine priming followed by VTT △C8-K3-gag boosting twice is the best immunization strategy in our studies.

关 键 词：痘苗病毒天坛株 DNA疫苗 VTT△C8-K3-gag 免疫策略 

分 类 号：R392.11[医药卫生—免疫学]