拟线粒体促凋亡多肽N7在人卵巢癌细胞对顺铂增敏作用中的研究  

Research on SmacN7 in sensibilization of cisplatin for huam novarian cancer cells

作　　者：张红岩[1] 李红雨[1] 高玉霞[1] 贾冬丽[1] 姚俊阁[1] 秦巧红[1] 马学玲[1] 

机构地区：[1]郑州大学第三附属医院妇产科,河南郑州450052

出　　处：《中国妇幼保健》2013年第15期2454-2458,共5页Maternal and Child Health Care of China

摘　　要：目的:探讨拟线粒体促凋亡多肽N7(SmacN7)能否增加人卵巢癌细胞对顺铂的敏感性及其与Caspase-3表达的关系。方法:通过细胞增殖抑制率测定及平板克隆形成试验研究顺铂单独应用组(A组),50μg/L SmacN7+顺铂组(B组)、100μg/L SmacN7+顺铂组(C组)及200μg/L SmacN7+顺铂组(D组)的细胞增殖抑制率和细胞克隆形成率;采用细胞免疫化学法检测上述分组中Caspase-3的表达。结果:单独应用顺铂时,24、48、72 h的增殖抑制率分别为(6.12±1.16)%、(13.47±1.15)%及(28.91±1.08)%。SmacN7(50～200μg/L)联合顺铂应用时,随着SmacN7浓度的增加或作用时间的延长,细胞增殖抑制率明显增加,药物作用24、48、72 h后增殖抑制率分别为(9.34±1.78)%～(49.81±2.11)%、(25.24±2.11)%～(65.54±2.27)%、(44.45±1.21)%～(82.36±2.19)%。肿瘤细胞存活率明显降低,分别为(25.13±0.52)%、(21.17±0.55)%、(18.23±0.54)%和(15.27±0.56)%。SmacN7联合顺铂应用后,Caspase-3的表达比单独应用顺铂明显增加(P=0.000)。结论:SmacN7能够明显增加人卵巢癌细胞对顺铂的敏感性。Objective： To explore whether SmacN7 can increase senxitivity of human ovarian cancer cells to cisplatin and its rela-tionship with expression of Caspase - 3. Methods： MTr assay and Flat cloning experiment were used to study the control group： eisplatingroup （group A, 50μg/L）, SmacN7 and cisplatin group （group B, 100 μg/L）, SmacN7 and cisplatin group （group C, 200 μg/L）, Sm- acN7 and eisplatin group （group D） , the inhibition rate of cell proliferation and cell colony formation rate were evaluated. Immunocytochem- istry was used to detect caspase -3 experssions. Results： When applying cisplatin alone, the proliferation inhibition rate at 24, 48 and 72 hours were （ 6. 12 ± 1.16 ） % , （ 13.47±1.15 ） % , and （ 28.91±1.08 ） % , respectively. When applying SmacN7 （ 50 - 200 μg/L） com- bined with cisplatin, with the increase of SmacN7 concentration or the prolongation of treatment time, the proliferation inhibitory rates of cancer cell increased, which were （9.34±1.78）% - （49.81 ±2.11）%, （25.24 ±2.11）% - （65.54±2.27）%, and （44.45±1.21） % - （82. 36±2. 19）% respectively at 24, 48, and 72 hours. The survival rates of tumor cell decreased significantly, which were （25. 13 ±0. 52）%, （21.17±0. 55）%, （18. 23 ±0. 54）%, and （15.27 ±0. 56）%, respectively. After applying SmaeN7 combined with eisplatin, the expression rate of Caspase - 3 increased statistically significantly, compared with applying cisplatin alone, there was statistical- ly significant difference when explore by Flat cloning experiments, there was statistically significant difference （ P = 0. 000） . Conclusion： SmacN7 can significantly increase the sensitivity of human ovarian cancer cells to cisplatin.

关 键 词：拟线粒体促凋亡多肽N7 Caspase-3卵巢癌顺铂 

分 类 号：R737.31[医药卫生—肿瘤]