利用SPR生物传感器检测牛奶中的氨苄青霉素残留  

Study on ampicillin residue detection in milk based on SPR biosensors

作　　者：刘瑾[1] 张婉洁[1] 王文[1] 徐可欣[1] 

机构地区：[1]天津大学精密测试技术及仪器国家重点实验室天津大学精密仪器与光电子工程学院,天津300072

出　　处：《化学研究与应用》2013年第5期754-759,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(30700146)资助

摘　　要：近年来,在食品安全检测领域,利用表面等离子体共振(surface plasmon resonance,SPR)传感器结合免疫测定方法进行痕量物质检测的技术得到了迅速发展。本文采用表面等离子共振生物传感器检测牛奶中的氨苄青霉素残留。从pH值、离子浓度和配体浓度三个方面讨论了实验条件对配体在传感器表面固定的影响,实验结果表明pH值为4.5、离子浓度为10mM以及配体浓度为400μg.mL-1为较佳实验条件。在此实验条件下对BSA-氨苄青霉素偶联物进行了固定,并采用该传感器对不同浓度的氨苄青霉素的水溶液和牛奶溶液进行了检测,最低检测限LOD分别为1.7ng.mL-1和1.8ng.mL-1,均低于氨苄青霉素的最大残留检测限(maximum residue limit,MRL)4.1ng.mL-1,验证了检测方法的可行性。In recent years,the novel technique for trace concentration detection based on surface plasmon resonance and immunoas- say was developed fast in food safety field. Surface plasmon resonance（SPR） biosensors were used to detect ampieillin residues in milk in this paper. First, the influence of some factors related to the immobilization of ligand on sensor surface was discussed, inclu- ding pH, ionic strength and concentration of ligand. Results of the experiment show that pH 4. 5, ionic concentration of 10mmol ~ L-~ ,and ligand concentration of 400μg·mL^-1 are optimum experiment conditions. And then, immobilizing the BSA-ampicillin on the SPR sensor surface under the above mentioned conditions, ampicillin solutions in water and milk with several concentrations were detected. The experiment results show that the limits of detection（LODs） are 1.7ng·mL^-1 and 1.8ng·mL^-1 respectively, which are lower than the maximum residues limit（ MRL）4. 1ng·mL^-1. So the detection method is feasible very well

关 键 词：抗生素残留 氨苄青霉素残留 表面等离子共振 生物传感器 竞争抑制法 

分 类 号：O657.99[理学—分析化学]