高压氧对孤独症鼠模型神经营养因子和小清蛋白表达的影响  

Effects of hyperbaric oxygen on the level of brain derived neurotrophic factor and parvalbumin in hippocampal CA1 region of vaiproic acid autism rats

作　　者：衣明纪[1] 冯雪英[1] 杨召川[1] 付伟伟[2] 李音[3] 冉霓[1] 傅芃[1] 

机构地区：[1]青岛大学医学院附属医院儿童保健科,266003 [2]青岛大学医学院附属医院病理科,266003 [3]青岛市妇女儿童医疗保健中心儿童保健科

出　　处：《中华实用儿科临床杂志》2013年第7期542-545,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：青岛大学医学院附属医院2010年院内重点培育学科专项建设基金项目（610）

摘　　要：目的探讨高压氧对丙戊酸钠（VPA）孤独症模型鼠海马CAl区脑源性神经营养因子（BDNF）和小清蛋白（PV）表达水平的影响，以阐明高压氧治疗孤独症的可能机制。方法按Schneider和Przewlocki的方法制作vPA孤独症动物模型，Wistar大鼠怀孕第12．5天腹腔注射600mg／kgVPA后所产下的子代雄鼠，根据子鼠睁眼时间、行为表现、断乳时体质量，结合生后第28天采用Y型电迷宫测试其学习记忆能力，获得48只造模成功的孤独症模型雄鼠，随机分为高压高氧模型组、高压空气模型组、常压高氧模型组、常压空气模型组，每组12只；正常对照组为Wistar孕鼠同期腹腔注射等量9g/L盐水后所产下的子代雄鼠12只（常压空气正常组）。采用免疫组织化学和图像分析技术检测各组鼠海马CAl区BDNF和PV表达水平。结果常压空气模型组与常压空气正常组比较，海马CAl区BDNF阳性细胞数增加（5．00±1．60比3．00±1．04，t=3．633，P=0．001）；PV阳性细胞数增加（5．33±0．99比2．83±1．29，t=5．369，P=0．000）。高压高氧模型组与常压空气模型组比较，海马CAl区PV阳性细胞数减少（3．33±0．99比5．33±0．99，t=4．975P=0．000）。高压高氧模型组与高压空气模型组比较，海马CAl区PV阳性细胞数减少（3．33±0．99比4．67±1．92，t=-2．138，P=0．044）。结论孤独症的发病可能与海马CAl区BDNF和PV的表达水平有关，高压氧干预可能通过减少CAl区神经元PV的表达发挥作用。Objective To explore the outcome of brain derived neurotrophic factor（BDNF） and parvalbumin （PV） positive neurons after hyperbaric oxygen intervention for the valproie acid（VPA） autism rats in hippocampal CA1 region. Methods The animal model of autism was established by the methods of Schneider and Przewloeki. Animal model groups of autism were obtained in male offspring of the Wistar rats that received intraperitoneal injection of 600 mg/kg VPA on the 12.5 day after pregnancy according to the eye opening time, behavior,weaning weight and the learning and memory abilities which were evaluated by the Y electricity maze test on the 28th day after birth. Forty-eight male VPA autism rats were randomly divided into high-pressure high-oxygen group, high-pressure air group and normal pressure high-oxygen group and normal pressure air group（ n = 12, each group）. Normal control group was obtained in male offspring of Wistar rats that received intraperitoneal injection of equivalent physiological saline in the same period （n = 12）. Using the immunohistochemistry methods and image analysis to examine the number of BDNF and PV positive neurons in hippocampal CA1 region of different groups. Results The number of BDNF positive neurons in normal pressure air model group was more than that in the normal pressure air control group ,with statistical difference between them（5.00 ± 1.60 vs 3. 00 ±1. 04,t =3. 633 ,P =0. 001 ）. The number of the PV positive neurons in the normal pressure air model group was more than that of the normal pressure air control group, with statistical difference between them （5.33±0.99 vs 2.83± 1.29, t = 5. 369, P = 0. 000）. The number of the PV positive neurons in the high-pressure high-oxygen group was less than that in the normal pressure air model group, with statistical difference between them （ 3.33± 0.99 vs 5.33 ± 0.99, t = 4. 975,P = 0. 000）. The number of the PV positive neurons in the high-pressure high- oxygen group was less than that in the high-pressure-ai

关 键 词：孤独症 高压氧 丙戊酸钠 脑源性神经营养因子 小清蛋白 大鼠 

分 类 号：R-3[医药卫生] R74