毛栓菌产漆酶条件优化及该酶对合成染料脱色的特性  

The optimization on laccase production from Trametes hirsuta and its property in the decolorization of synthetic dyes

作　　者：刘文华[1] 蔡宇杰[1] 范晶晶[1] 廖祥儒[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《微生物学通报》2013年第5期727-738,共12页Microbiology China

基　　金：国家863计划项目(No.2010AA101501);重大科技支撑与自主创新专项引导资金项目(No.BY2010117);无锡市科技支撑计划项目(No.CSE01N1210)

摘　　要：【目的】提高菌株Trametes hirsuta SYBC-L19漆酶产量,并研究该酶对合成染料脱色的性质。【方法】通过单因素和响应面设计,对产漆酶培养基进行优化。【结果】最优培养基为:玉米粉20.0 g/L、马铃薯淀粉32.4 g/L、酒石酸铵2.9 g/L、吐温80 0.5 g/L、CuSO4.5H2O 2.0 mmol/L、香兰素0.54 mmol/L、NaH2PO4.2H2O 2.0 g/L、MgSO4.7H2O0.5 g/L、MnSO4.H2O 0.1 g/L;最佳培养条件为:培养温度30°C,初始pH 6.0,装液量40 mL/250 mL,接种量8%。【结论】培养8 d酶活达35 U/mL,是优化前的39倍。对漆酶催化合成染料脱色进行了考察,发现该酶在60°C下对偶氮类染料AR1和RB5能迅速脱色,5 min内即可完成。To improve laccase production and apply it to decolorize synthetic dyes, we made this research. [Methods] The nutritional and environmental conditions for laccase production were investigated. [Results] The optimum conditions described as follows： corn flour 20.0 g/L, potato starch 32.4 g/L, ammonium tartrate 2.9 g/L, Tween 80 0.5 g/L, CuSO4？5H2O 2.0 mmol/L, vanilline 0.54 mmol/L, NaH2PO4？2H2O 2.0 g/L, MgSO4？7H2O 0.5 g/L, MnSO4？H2O 0.1 g/L, inoculation concentration 8%, pH 6.0, liquid volume 40/250 mL, culture temperature 30 °C. [Conclusion] With the conditions, the activity of laccase was achieved at 35 U/mL, was higher 39 times than control. The laccase was applied to decolorize synthetic dyes and it was found that the laccase could decolorize azo dyes AR1 and RB5 rapidly which could be completed in 5 minutes.

关 键 词：TRAMETES hirsuta SYBC-L19 漆酶 优化 响应面 脱色 

分 类 号：TQ925[轻工技术与工程—发酵工程]