环境水体中纳米氧化铜对金鱼藻的毒性效应研究  

Toxic Effects of Low Concentration Copper Oxide Nanoparticles on Ceratophyllum demersum in the Aquatic Environment

作　　者：崔静[1] 袁旭音[1] 刘泉[1] 郭荣荣[1] 

机构地区：[1]河海大学环境学院,南京210098

出　　处：《农业环境科学学报》2013年第5期910-915,共6页Journal of Agro-Environment Science

基　　金：国土资源公益性行业科研专项经费项目(201111021);河海大学自然科学基金项目(2008432511)

摘　　要：采用模拟水体环境的方法,研究了低浓度（2~8 mg.L-1）纳米氧化铜（CuONPs）溶液对金鱼藻生长的毒性影响,并与相同浓度的微米氧化铜和可比剂量铜离子进行对比分析。实验结果显示在纳米氧化铜溶液的作用下,金鱼藻对Cu元素的积累量为相同浓度的微米氧化铜溶液作用下的6~12倍,金鱼藻体内的酶活性对纳米氧化铜溶液作用的浓度和时间均有明显反应。在作用20 d时,4mg.L-1的纳米氧化铜溶液作用下金鱼藻体内POD、SOD活性均达到最大值;在作用30 d时,同样浓度作用下金鱼藻的叶绿素含量达到峰值,其后逐步下降。对于8 mg.L-1纳米氧化铜溶液的可比剂量铜离子（0.23 mg.L-1）作用于金鱼藻,其POD活性、SOD活性和叶绿素含量的变化明显小于8 mg.L-1浓度的纳米氧化铜溶液。上述结果表明低浓度（4~8 mg.L-1）的纳米氧化铜溶液对金鱼藻生长有明显的抑制效应和毒害作用,纳米氧化铜对金鱼藻的生长表现出很强的纳米效应,其生长同时受到纳米氧化铜溶液作用时间的影响,但低浓度的微米氧化铜溶液对金鱼藻生长几乎不存在毒害作用。A solution experiment of simulated water environment was conducted to study the toxic effects of low concentration(2~8 mg·L-1)copper oxide nanoparticles(CuONPs) on Ceratophyllum demersum.The same concentrations of copper oxide micronparticle(CuOMPs) and the Cu2+ corresponding to CuONPs were compared.The experimental results showed that the concentrations of Cu in Ceratophyllum demersum growing in CuONPs solution were about 6~12 times than the concentrations exposing in CuOMPs solution.When Ceratophyllum demersum were cultured for 20 d,the activities of POD and SOD reached the maximum values under the stress of 4 mg·L-1 CuONPs solution and when the Ceratophyllum demersum were cultured for 30 d,the chlorophyll-a level reached a peak at the same concentration.Under the stress of comparable Cu2+ concentration(0.23 mg·L-1) of 8 mg·L-1 CuONPs,the activities of POD and SOD,the chlorophyll-a level were significantly less than those influenced by 8 mg·L-1 CuONPs solution.These results indicated that the CuONPs solution of low concentrations(4~8 mg·L-1) had the restraining or toxic effects on the growth of Ceratophyllum demersum.The CuONPs solution exhibited the remarkable ecological effects with nanoparticle characters,as well as the time scale effects for the growth of Ceratophyllum demersum.But the CuOMPs solution of low concentration had no observed toxic effects on Ceratophyllum demersum.

关 键 词：纳米氧化铜 金鱼藻 抗氧化酶 叶绿素 毒性效应 

分 类 号：X503.233[环境科学与工程—环境工程]