精噁唑禾草灵降解菌株Acinetobacter sp.T-1的分离鉴定及降解特性研究  

Isolation and Characterization of Fenoxaprop-p-ethyl-Degrading Bacteria Strain Acinetobacter sp.T-1 and Its Degrading Characteristics

作　　者：董维亮[1] 侯颖[1,2] 陶健[1] 曹慧[1] 崔中利[1] 

机构地区：[1]南京农业大学生命科学学院,农业部农业环境微生物重点开放实验室,南京210095 [2]河南科技大学食品与生物工程学院,河南洛阳471003

出　　处：《农业环境科学学报》2013年第5期979-985,共7页Journal of Agro-Environment Science

基　　金：国家自然科学基金(31270095);江苏省杰出青年基金(BK2012029);国家科技支撑计划(2012BAD14B02);农业部"948"项目

摘　　要：从长期受精噁唑禾草灵污染的土壤中分离筛选得到了精噁唑禾草灵降解菌T-1,根据生理生化特性和16S rRNA同源性序列分析,将其鉴定为不动杆菌属（Acinetobacter sp.）。菌株T-1能够以精噁唑禾草灵为唯一碳源进行生长,在5 d内对50 mg.L-1精噁唑禾草灵的降解率可达95%以上。T-1降解精噁唑禾草灵的最适温度为37℃,而其在pH5~11的范围内对50 mg.L-1精噁唑禾草灵的降解率均可以达到85%以上。经LC-MS鉴定Acinetobacter sp.T-1降解精噁唑禾草灵的主要产物为精噁唑禾草灵酸,表明菌株T-1对精噁唑禾草灵的降解是通过断裂其酯键形成精噁唑禾草灵酸和乙醇实现的。Fenoxaprop-p-ethyl(FE) is a postemergence applied aryloxyphenoxy propionate(AOPP) herbicide used for the control of annual and perennial weeds in crops and it caused severely environmental pollution for its widely used in agriculture.Bioremediation is an effective and economic method to treat the environment that has been polluted by FE.So researchers paid much attention in this area.In the present study,a bacterial strain T-1 capable of degrading FE was isolated from the soil which was contaminated by FE for a long time.Strain T-1 was preliminarily identified as Acinetobacter sp.based on its physiological and biochemical characters and 16S rRNA gene sequence analysis.Strain T-1 could utilize FE as the sole carbon source for growth,and degrade 95% of 50 mg·L-1 FE within 5 days.The optimal temperature of FE degradation by strain T-1 was 37 ℃,and stain T-1 could degrade over 85% of 50 mg·L-1 FE within pH5~11.Inoculum size and concentration of substrate had a significant effect on the degradation of FE by strain T-1.And the degradation rate could increase with the increasing of the inoculum size,while the increase in the concentration of the substrate would inhibit the degradation of FE.The ion Ni2+ could inhibit the strain to degrade FE slightly,and other metal ions had no effect on the degradation of FE by strain T-1 in the experimental range.One major metabolite fenoxaprop acid(FA) was identified by LC-MS during the degradation of FE and this showed that the metabolic pathway of strain T-1 degrading FE was the cleavage of ester bonds to form FA and ethanol.

关 键 词：精噁唑禾草灵 降解 Acinetobactersp 

分 类 号：X172[环境科学与工程—环境科学]