HPLC同时测定消臌胶囊中没食子酸和橙皮苷的含量  

Simultaneous determination of gallic acid and hesperidin contained in Xiaogu capsule by HPLC

作　　者：林菲菲[1] 谢文兵[2] 于杰[3] 许维国[1] 牛凤兰[1] 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]中国科学院应用化学研究所,吉林长春130021 [3]吉林大学中日联谊医院,吉林长春130021

出　　处：《中国中药杂志》2013年第10期1518-1520,共3页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2011CB935800);吉林省科技厅重点项目(20100905);吉林省高技术产业发展项目(2010014)

摘　　要：目的:建立同时测定消臌胶囊中没食子酸和橙皮苷的HPLC法,为该中药复方制剂的质量控制提供一种简便、快速、准确的方法。方法:采用甲醇加热回流提取消臌胶囊,选取Synergi 4μHydro-RP 80A色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.04 mol.L-1磷酸二氢钠溶液(20∶80),流速1.0 mL.min-1,检测波长283 nm,柱温25℃。结果:在此色谱条件下,没食子酸和橙皮苷达到基线分离,分别在21.6～216.0,4.5～45.0 mg.L-1与峰面积成良好的线性关系,平均回收率(n=9)分别为101.5%(RSD 3.7%),94.7%(RSD 2.7%)。消臌胶囊中没食子酸和橙皮苷的平均含量分别为5.10%,0.091 1%。结论:该研究建立的方法能快速准确地测定消臌胶囊中没食子酸和橙皮苷的含量,可为该药的质量评价提供参考。Objective： To develop an HPLC method for simultaneous determination of gallic acid and hesperidin in Xiaogu capsule, in order to provide a simple, rapid and accurate method for quality control of the compound preparation of traditional Chinese medicine. Method： Xiaogu capsule was extracted with methanol heating reflux method. Synergi 4μ Hydro-RP 80A （4. 6 mm × 250 mm,5 μm） was adopted as the chromatographic column, with acetonitrile-0. 04 mol ~ L-1phosphate monobasic sodium solution （20： 80） as the mobile phase. The flow rate was 1.0 mL· min^- 1, the detection wavelength was 283 nm, and the column temperature was 25 ℃. Result： Under the conditions, gallic acid and hesperidin reached the baseline resolved peak, with a good linearity within the range of 21.6-216.0 mg· L^-1 （ r = 0. 999 93 ） for gallic acid, and 4. 5-45.0 mL·min^-1 （ r = 0. 999 95 ） for hesperidin, respectively. Their average recoveries （ n = 9） were 101.5% （ RSD 3.7% ） and 94. 7% （ RSD 2. 7% ）, respectively. The average contents of gallic acid and hesperidin contained in Xiaogu capsule were detected to 5.10% and 0. 091 1%, respectively. Conclusion： The method established in this study can determine the content of gallic acid and hesperidin contained in Xiaogu capsule in a rapid and accurate manner, which provided reference for quality evaluation of the medicine.

关 键 词：HPLC 没食子酸 橙皮苷 

分 类 号：R286[医药卫生—中药学]