商陆提取物醋制前后毒性作用的比较研究  

Comparative study on toxicity of extracts from Phytolaccae Radix before and after being processed with vinegar

作　　者：宫乐[1] 吴皓[1,2,3] 郁红礼[1,2,3] 赵腾斐[1] 徐秋香[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210029 [2]江苏省中药炮制重点实验室,江苏南京210029 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029

出　　处：《中国中药杂志》2013年第10期1610-1613,共4页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局公益性行业专项基金项目(200807039)

摘　　要：目的:提取分离获得商陆的毒性成分,并比较其醋制前后毒性的变化。方法:采用黏膜刺激性反应、小鼠腹腔炎症模型及离体巨噬细胞释放NO模型,比较商陆毒性成分醋制前后的致炎毒性变化。结果:商陆毒性成分具有显著的致炎毒性,可导致家兔眼结膜水肿、小鼠腹腔渗出液中PGE2含量升高以及巨噬细胞释放NO含量升高;经醋制后,对家兔眼结膜刺激性减弱,使小鼠腹腔渗出液中PGE2含量降低、巨噬细胞释放NO含量降低。结论:醋制法能够降低商陆毒性成分的毒性作用。Objective： To extract and separate toxic components from Phytolaccae Radix, and to comare the changes in toxicity of Phytolaccae Radix before and after being processed with vinegar. Method： The mucous membrane irritation response, mouse peritoneal inflammation model and in vitro macrophages release NO model were applied to compared the changes in inflammatory toxicity of toxic components from Phytolaccae Radix before and after being processed with vinegar. Result： Toxic components of Phytolacca Radix had significant inflammatory toxicity, which could cause conjunctival edema in rabbits, and increase of PGE2 and macrophages release NO content in peritoneal exudate in mice. After being processed with vinegar, they showed reduced irritation, which resulted in decrease of PGE2 and macrophages release NO content in peritoneal exudate in mice. Conclusion： After being processed with vinegar, the toxicity of toxic components from Phytolacca Radix decreased obviously.

关 键 词：商陆 毒性成分 黏膜刺激性 炎症 

分 类 号：R285[医药卫生—中药学]