Survivin基因在阿糖胞苷诱导K562细胞凋亡机制中的研究  

Research on survivin gene in the apoptosis mechanism of K562 induced by cytarabine

作　　者：刘春艳[1,2] 王莉[1,2] 柴晓静[2] 李娟[2] 赵丽[2] 

机构地区：[1]兰州大学第一临床医学院 [2]兰州大学第一医院中心实验室,兰州730000

出　　处：《中国优生与遗传杂志》2013年第5期22-24,21,共4页Chinese Journal of Birth Health & Heredity

基　　金：甘肃省科技厅科技支撑计划;项目编号:1011FKCA129

摘　　要：目的探讨Survivin基因在阿糖胞苷诱导K562细胞凋亡过程中表达的变化及其在凋亡途径中与Caspase-8、Caspase-9、Caspase-3 mRNA表达的相关性。方法 MTT比色法检测K562细胞对阿糖胞苷的敏感性,以阿糖胞苷的IC50药物浓度处理K562细胞;流式细胞术检测各组细胞凋亡率;Western blot方法检测各组Survivin蛋白质的表达;实时荧光定量PCR检测各组细胞Survivin、Caspase-8、Caspase-9、Caspase-3mRNA表达。结果 Survivin mRNA及蛋白表达在阿糖胞苷处理24、48、72h后较对照组明显降低(P<0.05),与细胞凋亡呈负相关;Caspase-8、Caspase-9、Caspase-3mRNA经阿糖胞苷处理24、48、72h后比对照组明显增高(P<0.05),与细胞凋亡呈正相关。结论阿糖胞苷可诱导K562细胞凋亡,其机制可能与抑制Survivin基因表达,上调Caspase-8、Caspase-9、Caspase-3 mRNA有关。Objective： To discuss the change of the expression of Survivin in the apoptosis mechanism of K562 cells induced by Cytarabine and the expression correlation with the expression of the mRNA of Caspase - 8, Caspase - 9 and Caspase - 3. Methods ： MTT was used to test the sensibility of K562 cell to Cytarabine, The ICS0 of Cytarabine was chosen by MTI assay, FCM was used to test apoptosis rate of K562, Real time - PCR was applied to detect the expression of Survivin, Caspase - 8, Caspase - 9, caspase - 3 mRNA. Western blot was provided to check the expression of Survivin protein. Results： Compared with control group, the level of Survivin was lower after exposed to Cytarabine for 24 hours, 48 hours and 72hours （ P 〈 0. 05 ） and negatively correlated with apoptosis rate of K562, the expression of Caspase - 8, caspase - 9, Caspase - 3 mRNA were higher after 24 hours, 48 hours and 72 hours （ P 〈 0. 05 ） and positive correlation with apoptosis rate of K562. Conclusions ： Cytarabine can effectively induce apoptosis of K562 cells, and its mechanism may be related with the inhibition of Survivin expression and the up - regulation of the mRNA expression of Caspase - 8, Caspase - 9 and Caspase - 3 .

关 键 词：Survivin K562细胞 CASPASE-8 CASPASE-9 Caspase-3 凋亡 

分 类 号：R363[医药卫生—病理学]