慢病毒载体介导的RNA干扰下调膜联蛋白A1对小细胞肺癌细胞生物学行为的影响  

The effect of down regulation of AnnexinA1 by lentiviral vector-mediated RNAi on the biological behavior of small-cell lung cancer cell lines

作　　者：周菁[1] 张峰[1] 庞海林[1] 马丽[1] 刘佳钰[1] 刘理礼[1] 张贺龙[1] 

机构地区：[1]第四军医大学唐都医院肿瘤科,陕西西安710038

出　　处：《现代肿瘤医学》2013年第5期917-921,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81172001)

摘　　要：目的:探究AnnexinA1对小细胞肺癌细胞生物学行为的影响。方法:构建干扰AnnexinA1的慢病毒载体,转染至SBC-5细胞株;体外增殖、迁移、侵袭实验观察下调AnnexinA1表达对小细胞肺癌细胞株生物学行为的影响。结果:干扰AnnexinA1的细胞株ANXA1 RNAi-SBC-5构建成功。与转染空载体的对照细胞相比,抑制AnnexinA1的表达能够显著抑制SBC-5细胞的增殖、迁移、侵袭能力。结论:AnnexinA1表达可能与肺癌骨转移密切相关。Objective：To explore the effect of AnnexinA1 on the biological behavior of small cell lung cancer cell lines. Methods：The expression of AnnexinA1 was down- regulated by RNA inference （RNAi） in SBC -5 cells. MTl＇,in vitro scratch test ,transwe11 based methods were used to detect the effect of AnnexinA1 on the proliferation, migration and invasion ability of small cell lung cancer cells. Results：The cells down - regulated the expression of AnnexinA1 were constructed. Down regulation of AnnexinA1 can decrease the proliferation ,migration and invasion potential in SBC -5 cell lines. Conclusion：The expression of AnnexinA1 is closely related to bone metastasis.

关 键 词：骨转移 膜联蛋白A1 慢病毒载体 小干扰RNA 小细胞肺癌 

分 类 号：R73-362[医药卫生—肿瘤] R734.2[医药卫生—临床医学]