BRCA1 mRNA表达与结直肠癌标准方案化疗临床预后相关性  

Relationship of BRCA1 mRNA expression and prognosis of colorectal cancer patients received the standard first-line chemotherapy

作　　者：张一帆[1,2] 禹立霞[2] 张广亮[2] 沈洁[2] 胡静[2] 王立峰[2] 杨阳[2] 钱晓萍[1,2] 刘宝瑞[2] 

机构地区：[1]南京医科大学鼓楼临床医学院肿瘤中心,江苏南京210008 [2]南京大学医学院附属鼓楼医院肿瘤中心,江苏南京210008

出　　处：《现代肿瘤医学》2013年第5期1070-1074,共5页Journal of Modern Oncology

基　　金：江苏省卫生厅科技发展基金(编号:201035)

摘　　要：目的:探讨结直肠癌组织中乳腺癌易感基因(breast cancer susceptibility gene1,BRCA1)mRNA表达水平与接受标准方案化疗的患者临床病理的关系及预后意义。方法:采用荧光定量PCR方法检测福尔马林固定-石蜡包埋的结直肠癌组织中BRCA1 mRNA的表达水平,并分析其表达水平与接受标准方案化疗的患者的临床病理及生存期之间的关系。结果:BRCA1 mRNA表达水平与患者淋巴结转移(P=0.008)、脉管侵犯(P=0.013)统计学意义有相关性,与其余肿瘤病理特征无相关性。BRCA1 mRNA中高表达组患者经标准方案化疗后平均总生存期为69.56个月,低表达组平均总生存期为55.67个月,两组间总生存期的差异(P=0.039)具有统计学意义,无进展生存期差别(P=0.93)没有统计学意义。性别(P=0.023)、分期(P<0.001)、以肠梗阻或肠穿孔起病(P=0.046)是患者总生存期的独立影响因素;年龄(P=0.043)、分期(P<0.001)、脉管侵犯(P=0.002)是患者无进展生存期的独立影响因素。结论:BRCA1 mRNA的表达水平可能是使用标准方案化疗的结直肠癌患者预后的影响因素。性别、分期、以肠梗阻或肠穿孔起病、年龄、脉管侵犯可能是影响结直肠癌患者预后的独立预测因素。Objective：To investigate the correlation of the mRNA expression levels of the breast cancer susceptibility genel （BRCA1）with clinicopathological parameters in patients with colorectal cancer receiving the standard first - line chemotherapy regimens as well as their clineal prognostic significance. Methods ： The mRNA expression of BRCA1 in formalin - fixed paraffin - embeded colorectal cancer tumor specimens was measured using quantitative reverse transcription polymerase chain reaction. And the relationship between BRCA1 levels with elinicopathological parameters and survival in colorectal cancer was studied. Results：BRCA1 expression was not related to clinieopathological parameters except lymphnode matastasis（ P = O. 008 ） and vessel invasion（ P = 0.013 ） in eolorectal cancer, the time of overall survival in middle -high group of BRCA1 mRNA expression was 69.56 months ,low group was 55.67 months. BRCA1 mRNA hypoexpression was a significantly favorable factor for overall survival （ P = 0.039 ） , not for time to progression （ P = 0.93 ）. From Cox proportional hazards model for survival, sex （ P = 0.023 ）, stage （ P 〈 0.001 ）, intestinal obstruction or perforation as initial symptom（ P = 0.046）were independent predictive markers of overall survival of colorectal cancer, age （ P = 0.043 ）, stage （ P 〈 0.001 ）, vessel invasion （ P = 0.002 ） were independent predictive markers of progression free survival. Conclusion： It was suggested that intratumoral BRCA1 mRNA expression level maybe a predictive marker of survival of the standard first - line chemotherapy regimens in colorectal cancer. Sex, stage, intestinal obstruction or perforation as initial symptom, age, vessel invasion were independent predictive markers of colorectal cancer.

关 键 词：BRCA1 结直肠癌 化疗 标准方案 

分 类 号：R735.3[医药卫生—肿瘤]