Snail在卵巢癌细胞侵袭转移潜能方面研究  

Role of Snail in the Invasion and Migration of Ovarian Carcinoma Cells

作　　者：夏曦[1,2] 蒋学峰[1,2] 纪腾[2] 徐洪斌[2,3] 

机构地区：[1]广东医学院附属深圳市南山区人民医院妇产科,广东省深圳市518052 [2]华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉市430030 [3]暨南大学附属深圳市人民医院妇产科,广东省深圳市518020

出　　处：《医学分子生物学杂志》2013年第1期21-25,共5页Journal of Medical Molecular Biology

基　　金：广东省自然科学基金（No.S2011040006012）,广东省医学科研基金（No.B2011295）,深圳市科技计划（No.20110422597;No.201002006）,深圳市南山区科技计划（No.2010028）

摘　　要：目的研究Snail与卵巢癌侵袭转移问的关系，并探讨其分子机制。方法分别构建正义全长Snail真核表达载体和小分子干扰RNA，分别转染卵巢癌细胞系A2780、C13＊。采用Western印迹方法分析相关蛋白表达，Transwell试验检测细胞侵袭转移能力。结果①成功构建Snail正义全长真核表达载体，并合成Snail小分子RNA干扰片段；②在转染PEGFPCI／Snail的卵巢癌细胞株A2780、C13＊中E-eadhefin表达明显下调，Snail和Vimentin则表达上调；而在转染Snail／SiRNA的卵巢癌细胞株A2780、C13‘中E—cadherin表达明显上调，Snail和Vimentin则表达下调；③Snail过表达可显著促进卵巢癌细胞株A2780、C13＊的侵袭转移潜能；封闭Snail表达水平可一定水平抑制卵巢癌细胞株A2780、C13＊的侵袭转移潜能。结论snail可通过促进卵巢癌上皮细胞间质化转变（epithelial—mesenchymaltransition，EMT）而提高其侵袭转移能力；封闭Snail表达可一定程度逆转卵巢癌上皮细胞间质化转变（EMT）而抑制其侵袭转移能力。Objective To examine the relationship between Snail and the invasion and migra- tion of ovarian carcinoma cells and the underlying molecular mechanism. Methods The eukaryotic expression vector carrying full-length sense Snail gene and the Snail-targeting small molecule RNA interference fragment were constructed and transfected into A2780 and C13 ＊ cells （two ovarian car- cinoma cell lines） respectively. Western blotting was used to detect the expression of Snail, E-cad- herin and Vimentin. The invasive and migratory ability of cells was examined by transwell as- say. Results The Snail over-expressing plasmid PEGFPC1/Snail and the small molecule RNA in- terference fragment Snail/siRNA were successfully constructed. After the PEGFPC1/Snail transfec- tion, the expression level of E-cadherin was significantly decreased and that of Snail and Vimentin substantially increased in A2780 and C13 ＊ cells. Transfection with Snail/SiRNA resulted in an in-crease in the expression of E-cadherin and a decrease in the expression of Snail and Vimentin in A2780 and C13 ＊ cells. Snail overexpression contributed to the invasion and migration of A2780 and C13 ＊ cells and Snail silencing inhibited such abilities of the cells. Conclusion Snail may enhance the potential of invasion and migration of ovarian carcinoma cells by inducing epithelial-mesenchymal transition （EMT） . Snail silencing may inhibit the cell invasion and migration by reversing the change of EMT.

关 键 词：转录因子SNAIL 卵巢癌侵袭转移 上皮间质变(EMT) e-cadherin VIMENTIN 

分 类 号：R737.33[医药卫生—肿瘤]