NOD/SCID小鼠中A431侧群与非侧群细胞成瘤组织中EMT相关分子的表达  

Expression of epithelial-mesenchymal transition markers in tumor tissues generated by side population and non-side population of A431 cells in NOD/SCID mice

作　　者：邵玲巧[1] 王倩倩[2] 郭圆圆[2] 耿松梅[2] 

机构地区：[1]西安医学院病原生物学与免疫教研室,陕西西安710021 [2]西安交通大学第二附属医院皮肤科,陕西西安710004

出　　处：《南方医科大学学报》2013年第5期733-737,共5页Journal of Southern Medical University

基　　金：教育部新世纪人才支持计划(NCET-10-0673);中央高校基本科研业务费专项基金;西安交通大学第二附属医院科研重点项目[YJ(ZD)201219]

摘　　要：目的旨在分离A431中侧群(SP)细胞及非侧群(NSP)细胞亚群,比较二者在小鼠皮下成瘤能力差异及组织中上皮间质转化(EMT)相关分子表达。方法培养A431细胞经荧光染料Hoeehst33342染色后,流式细胞仪分选出SP细胞和NSP细胞,接种到NOD/SCID小鼠皮下,观察各自成瘤能力,取瘤体组织免疫组织化学染色检测EMT相关分子E-Cadherin、β-catenin、Vimentin、AXL和Erbb3的表达。结果 A431细胞系中成功分选出SP细胞,小鼠皮下成瘤迅速。在SP细胞小鼠成瘤组织周围细胞中,E-Cadherin、Erbb3表达降低,β-catenin、Vimentin、AXL表达增高。结论 A431细胞中含有SP细胞亚群,该亚群细胞具有较强的肿瘤形成能力,且显示较强的EMT特性。Objective To isolate the side population （SP） and non-side population （NSP） cells from A431 cells and compare their difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition （EMT） markers. Methods A431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL, and Erbb3 in the tumor tissues were detected by immuohistostaining. Results The tumors generated by SP cells were larger than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3 and E-cadherin. Conclusion The SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in tissues.

关 键 词：鳞癌 侧群细胞 上皮间质转化 肿瘤干细胞 

分 类 号：R730.2[医药卫生—肿瘤]