运动训练对局灶性脑缺血大鼠神经功能及Sema3A表达的影响  

EFECTS OF EXERCISE TRAINING ON NEURAL FUNCTION AND EXPRESSION OF SEMA3A IN RATS WITH FOCAL CEREBRAL ISCHEMIA

作　　者：张振燕[1] 王强[1] 郭云良[2] 姚如永[3] 

机构地区：[1]青岛大学医学院附属医院康复一科,山东青岛266021 [2]青岛大学医学院附属医院脑科研究所,山东青岛266021 [3]青岛大学医学院附属医院中心实验室,山东青岛266021

出　　处：《齐鲁医学杂志》2013年第2期129-131,135,共4页Medical Journal of Qilu

基　　金：山东省教育厅科研发展计划(J09LF22)

摘　　要：目的探讨运动训练对局灶性脑缺血大鼠神经功能及Sema3A表达的影响。方法选取健康雄性SD大鼠30只,随机分为假手术组、对照组、训练组,每组10只。训练组采用线栓法建立左侧大脑中动脉阻塞2h再灌注动物模型,假手术组造模方法同训练组但不阻塞大脑中动脉血流。训练组大鼠于再灌注24h后游泳训练30min,共计3d;假手术组和对照组大鼠不行游泳训练。3d后对各组大鼠进行受损神经功能(MENZIES评分)、抓握力及平衡功能评定;处死大鼠,取缺血侧大脑皮质和海马,均用RT-PCR方法检测Sema3A-mRNA表达。结果训练组大鼠MENZIES评分低于对照组,而高于假手术组,差异均有显著意义(F=177.75,q=19.49、6.00,P<0.05);训练组大鼠抓握力及平衡功能评分高于对照组,而低于假手术组,差异均有统计学意义(F=57.63、117.00,q=7.25～12.46,P<0.05)。训练组Sema3A-mRNA的表达量低于对照组,高于假手术组,差异均有统计学意义(F=62.873、78.095,q=8.35～9.10,P<0.05)。结论运动训练能降低轴突抑制因子Sema3A-mRNA的表达,从而促进轴突再生、改善受损神经功能。Objective To explore the effects of exercise training on neural function and expression of Sema3A in rats with focal cerebral ischemia(FCI).Methods A total of 30 male SD rats were evenly randomized to three groups as sham-operation group,control group and training group.A model of left middle cerebral artery occlusion for 2 hours was created by intraluminal thread method of rats in the training group;model in the sham-operation group was created by the method as used in the tra-ining group,except blocking the middle cerebral artery.The rats in training group were offered swimming training for 30 min 24 hours after reperfusion for 3 days;those in sham-operation and control groups received no swimming training.MENZIES score,grasp strength and equilibrium function were assessed,the rats were then executed,ischemic side cerebral cortex and hippocampus taken,the expression of Sema3A-mRNA detected by using RT-PCR.Results The score of MENZIES in the rats in training group was lower than that in the control,and higher than that in the training group,the differences being significant(F=177.75;q=19.49,6.00;P<0.05);the score of grasp and balance was higher than that in the control,and lower than that in the sham-operation group(F=57.63,117.00;q=7.25-12.46;P<0.05).The expression of Sema3A-mRNA in training group was lower than that in the control,but higher than sham-operation group(F=62.873,78.095;q=8.35-9.10;P<0.05).Conclusion Exercise training can reduce the expression of Sema3A-mRNA,thus promote the regeneration of axons and improve the function of damaged nerve.

关 键 词：运动疗法 脑梗死 大鼠 神经再生 

分 类 号：R743.31[医药卫生—神经病学与精神病学]