HBV对肝癌细胞中MIA2表达的影响  

Effect of HBV on MIA2 expressions in HCC cells

作　　者：姚文暾[1,2] 杨少奇[2] 杨力[2] 何芳[2] 杨花[1,2] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学附属总医院消化内科,银川750004

出　　处：《重庆医学》2013年第16期1804-1805,1808,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81060194)

摘　　要：目的探讨黑色素瘤抑制蛋白2(MIA2)在肝癌细胞中的表达水平与乙型肝炎病毒(HBV)感染状态的关系。方法培养人肝癌细胞株HepG2和持续表达乙肝病毒的人肝癌细胞株HepG2.2.15,采用细胞免疫组织化学和蛋白免疫印迹检测(Western blotting)2种细胞株中MIA2蛋白表达水平差异。结果 MIA2蛋白在HepG2.2.15细胞株中的表达水平较HepG2细胞株减低,差异有统计学意义。结论 MIA2蛋白在HBV阳性肝癌细胞中表达水平减低,提示HBV感染可能通过抑制MIA2的表达导致肝癌发生。Objective To investigate the relationship of the MIA2 expression in hepatoma cells with HBV infection status. Meth-ods Cultured HBV-negative and HBV-positive hepatoma cell lines HepG2 and HepG2.2.15 cells,to investigate MIA2 protein ex-pression differences in two cell lines by immunohistochemistry and Western blot analysis. Results The MIA2 protein expression level reduced in the HepG2.2.15 cell line compared with HepG2 cell line,the result was statistically difference. Conclusion MIA2 protein expression in HBV-positive liver cancer cells reduced. The results indicat a possibility that HBV infection may lead to liver cancer through inhibiting MIA2 expression.

关 键 词：MIA2 肝炎 乙型 肝肿瘤 免疫组织化学 印迹法 蛋白质 

分 类 号：R735.7[医药卫生—肿瘤]