无溶剂体系酶催化酯化反应合成1,3-甘油二酯的研究  

Enzymatic Esterifiaction of 1,3-Diacylglycerol from Oleic Acid and Glycerol in a Solvent Free System

作　　者：鲁珊[1] 黄健花[1] 王兴国[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2013年第4期358-361,共4页Journal of Food Science and Biotechnology

基　　金：国家"十二五"科技支撑计划项目(2011BAD02B04)

摘　　要：在无溶剂体系中以油酸、甘油为原料,用专一性脂肪酶中合成1,3-DAG,实验发现真空脱水可以有效的增加油酸的转化率。在真空脱水的条件下,探讨了反应底物摩尔比、酶量和温度等因素对油酸的转化率和1,3-DAG质量分数的影响。结果表明:油酸与甘油摩尔比为2∶1时,酶量为6%,温度为65℃时1,3-DAG质量分数达到62%。1,3-diglyceride was esterified by oleic acid and glycerol using Lipozyme RM IM in a solvent free system. The effects of substrate molar ratio,reaction temperature and enzyme loading on the conversion rate of oleic acid and yield of 1,3-DAG were discussed in this paper. The results were as follows：molar ratio of oleic acid and glycerol 2：1,reaction temperature 65 ℃ ,enzyme loading 6%, under these conditions,the 1,3-DAG yield was up to 62%.

关 键 词：酯化反应 1 3-DAG LIPOZYME RM IM 油酸 甘油 

分 类 号：TQ225.242[化学工程—有机化工]