反相高效液相色谱法检测生物胺的法医学应用初探  

The preliminary study on forensic application for determination of biogenic amines by RP-HPLC

作　　者：张艳林[1,2] 杨业全[1] 尹晓宏[1] 

机构地区：[1]广东警官学院,广东广州510230 [2]广东环境保护工程职业学院,广东佛山528000

出　　处：《中国法医学杂志》2013年第2期89-91,95,共4页Chinese Journal of Forensic Medicine

基　　金：广东省高校优秀青年创新人才培养计划项目(LYM11094)

摘　　要：目的采用反相高效液相色谱法(RP-HPLC)检测大鼠肌肉组织中7种生物胺,并探讨其在法医学中的应用价值。方法以丹磺酰氯为衍生剂,通过ZORBAX Rx-C18反相色谱柱分离,以乙腈-水作流动相梯度洗脱,流速为1.0mL/min,柱温为20℃,DAD检测器,对色胺、腐胺、尸胺、组胺、酪胺、亚精胺、精胺7种生物胺进行检测。结果 7种生物胺得到良好分离,在0.5～100.0μg/mL的范围内线性良好,相关系数均>0.99,检出限在0.14～0.50μg/mL之间;对大鼠肌肉样本的添加回收率在83.8%～100.6%之间,相对标准偏差均<11%。结论采用RP-HPLC法检测生物胺,方法简便、快速、灵敏,能够满足尸体组织中生物胺检测的需要。Objective An analytical method of RP-HPLC has been developed to assay biogenic amines in rat muscle tissue and to discuss its forensic application. Methods The samples were derivatized with dansyl chloride and then determined by RP-HPLC on the ZORBAX Rx-Cls reverse phase chromatographic columns. A gradient elution program with a mixture of acetonitrile and water was used at a flow rate of 1.0 mL/min. The diode array detector （DAD） was used and the column temperature was 20℃. Results Seven kinds of biogenic amines were separated completely and the standard curve was linear over the concentration range of 0. 5 - 100. 01μg/mL. The detection limits were 0. 14 - 0. 50μg/mL for 7 kinds of biogenic amines. The average recoveries were in the range of 83.8% - 100.6% and the relative standard devitions were all less than 11%. Conclusion The method was convenient, rapid and sensitive, which could satisfy the deternination of biogenic amines in the organism.

关 键 词：法医毒物分析 生物胺 反相高效液相色谱 死亡时间 

分 类 号：D919[医药卫生—法医学]