褪黑素对6-OHDA诱导大鼠模型黑质神经元和胶质细胞的影响  

Experimental exploration on effect of melatonin on gliacytes and neurons in substantia nigra in rats with Parkinson disease

作　　者：阳桂香[1] 贾钰[2] 欧阳丽斯[2] 马宇昕[3] 李幽兰[2] 白海燕[1] 陈思[2] 李朝晖[1] 雷万龙[2] 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]中山大学中山医学院人体解剖学教研室,广东广州510080 [3]广东药学院基础学院人体解剖学教研室,广东广州510006

出　　处：《解剖学研究》2013年第2期85-89,F0004,共6页Anatomy Research

基　　金：国家自然科学基金(31070941;30770679);国家科委973项目(2010CB530000)分题(2010CB530004)

摘　　要：目的探察褪黑素(melatonin,MT)对6-羟基多巴(6-hydroxydopamine,6-OHDA)诱导的帕金森病(Parkinson’sdisease,PD)模型大鼠黑质神经元和胶质细胞的影响。方法选取35只成年雄性SD大鼠,随机分成正常对照组、6-OHDA处理组和6-OHDA+MT处理组,采用免疫组化染色检测各组大鼠黑质Iba1、GFAP阳性胶质细胞及多巴胺(Dopamine,DA)能神经元的形态结构变化,并进行细胞计数,蛋白印迹技术(Western blotting)检测TH、GFAP、OX42蛋白表达水平的变化。实验数据用统计学软件进行分析,P<0.05为差异具有统计学意义。结果 6-OHDA处理组黑质胶质细胞呈激活状态,且数量明显多于对照组和6-OHDA+MT处理组(P<0.05),TH阳性多巴胺能神经元数量明显少于对照组和6-OHDA+MT处理组(P<0.05),对照组和6-OHDA+MT处理组无明显差异(P>0.05);6-OHDA处理组TH蛋白的表达水平明显下调(P<0.05),OX42、GFAP蛋白的表达水平明显上调(P<0.05),对照组和6-OHDA+MT处理组无明显差异(P>0.05)。结论MT能够抑制6-OHDA帕金森病大鼠模型黑质胶质细胞的增殖和活化,并推测MT可能通过这一抑制作用保护DA能神经元。Objective To explore the protective effect of melatonin on gliacytes and neurons in substantia nigra in rats with Parkinson＇s disease induced by 6-hydroxydopamine （6-OHDA）. Methods Thirty five male adult SD rats were randomly divided into three groups： control group, 6-OHDA group and 6-OHDA＋MT group. Immunohistochemistry technique was used to detect the numberal and morpholoqical changes of fba-positive and GFAP- positive gliacytes and dopaminergic （DA） neurons. Western blot was used to detect the changes of expression of GFAP,OX42 and TH protein. Results Compared to control group and 6-OHDA＋ MT group, 6-OHDA group significantly had more activated gliacytes and less dopaminergic neurons （P〈0.05）, control group and 6-OHDA＋MT group showed no significantly difference （P〉0.05）. Expression of GFAP,OX42 in 6-OHDA group was significantly upregulated （P〈0.05） and TH protein expression was significantly downregulated （P〈0.05）. Control group and 6-OHDA＋MT group showed no significant difference （P〉0.05）. Coulusion Melatonin could inhibit the proliferation and activation of gliacytes in substantia nigra in rats with Parkinson＇s disease induced by 6-OHDA, and it might protect dopaminergic neurons via the inhibition.

关 键 词：帕金森病 褪黑素 黑质 6-羟基多巴 

分 类 号：R742.5[医药卫生—神经病学与精神病学]