2006年深圳市急性肠胃炎的流行病学和病毒学调查(英文)  

Epidemiological and virological investigation of acute gastroenteritis in Shenzhen, 2006

作　　者：谢飞飞[1,2] 何雅青[3] 程锦泉[3] 姜君[2] 杨洪[3] 赵金银[2] 张海龙[3] 姚相杰[3] 周俊宜[1] 吴伟立[2] 

机构地区：[1]中山大学中山医学院生物化学教研室,广东广州510080 [2]中国科学院北京基因组研究所,北京100101 [3]深圳市疾病预防控制中心,广东深圳518055

出　　处：《热带医学杂志》2013年第4期397-402,共6页Journal of Tropical Medicine

基　　金：Infectious Diseases Special Project,Minister of Health of China(2011ZX10004-001;2013ZX10004803)

摘　　要：目的研究2006年深圳市急性肠胃炎发病的主要病因。方法收集深圳急性肠胃炎粪便样本200份(包含122份散发样本和78份暴发样本),采用RT-PCR的方法进行急性肠胃炎相关病毒检测。结果 44%(88/200)的样本为诺如病毒阳性,9%(18/200)为轮状病毒A阳性,2%(4/200)为星状病毒阳性,2%(4/200)为肠道腺病毒阳性,还有8%(16/200)的样本为混合感染。其中散发样本中检出腺病毒、轮状病毒、星状病毒和诺如病毒GI、诺如病毒GII 5种病毒,而诺如病毒GII是3次暴发样本中的唯一病原。对22例NoV GII阳性样本测序后的系统发育分析表明NoV GII.4 variants 2006b cluster是造成2006年深圳市肠胃炎暴发的主要诱因,其次为NoV GII.12和GII.4 Hunter簇。结论 NoV GII.4是2006年流行于深圳的主要病毒株。Objective To determine the epidemiological and virological investigation of acute gastroenteritis in Shenzhen, 2006. Method Stool specimens of acute gastroenteritis （AGE） during 2006 from 122 sporadic cases and 78 outbreak cases were collected for AGE associated viruses detection by RT-PCR in Shenzhen, southern China. Results 44% （88/200） samples were detected positive for NoVs, 9% （18/200） for rotavirus A, 2% （4/200） for astrovirus and 2% （4/200） for enteric adenoviruses. 8% （16/200） were found with mixed infections of more than one virus. The sporadic cases were detected for the infection of adenovirus, rotavirns, astrovirus, NoV GI and GII wherever NoV GII was the only pathogen in three outbreaks. 22 samples positive for NoV GII were selected for sequencing. The results of phylogenetic analysis showed that NoV GII.4 variants 2006b cluster were responsible for most cases in Shenzhen in 2006, followed by NoV GII.12 and GII.4 Hunter cluster. Conclusion NoV GII.4 was the major strains circulating in Shenzhen in 2006.

关 键 词：急性肠胃炎 病毒学调查 诺如病毒 

分 类 号：R573.3[医药卫生—消化系统]