侵袭性牙周炎患者牙龈组织中MMP-8及TIMP-1的表达  

Expressions of MMP-8 and TIMP-1 in gingival tissue of patients with aggressive periodontitis

作　　者：岳新新[1,2] 薛蕊[3] 张炜[3] 刘娟[1,2] 轩东英[1,2] 章锦才[1,2] 赵红宇[1,4] 

机构地区：[1]南方医科大学附属口腔医院 [2]广东省口腔医院牙周科,广州510280 [3]郑州大学口腔医学院,郑州450052 [4]广东省口腔医院番禺分院牙周科,番禺511400

出　　处：《郑州大学学报（医学版）》2013年第3期366-369,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：广东省科技计划项目00204390134483040

摘　　要：目的:探讨基质金属蛋白酶-8(MMP-8)及其抑制因子-1(TIMP-1)在侵袭性牙周炎(AgP)牙龈组织中的表达情况。方法:采用免疫组织化学方法检测15例AgP患者和20例对照的牙龈组织中MMP-8和TIMP-1蛋白的表达情况。结果:MMP-8和TIMP-1在2组牙龈组织的上皮内及上皮下结缔组织中均有表达。AgP组MMP-8表达水平和MMP-8/TIMP-1均高于对照组(Z=2.990和4.911,P<0.05),2组TIMP-1的表达差异无统计学意义(Z=1.822,P>0.05)。ROC曲线分析结果显示,AUC(MMP-8)=0.752(95%CI=0.618～0.885,P=0.003),AUC(MMP-8/TIMP-1)=0.913(95%CI=0.831～0.996,P<0.001),AUC(TIMP-1)=0.347(95%CI=0.194～0.499,P=0.068)。结论:牙龈组织中MMP-8表达水平和MMP-8/TIMP-1可以作为诊断AgP的潜在指标。Aim ： To study the expression of matrix metalloproteinase-8 （MMP-8） and its inhibitory factor-1 （ TIMP-1 ） in gingival tissue of patients with aggressive periodontitis（AgP）. Methods： Immunohistochemistry method was used to de- tect MMP-8 and TIMP-1 protein in gingival tissue of 15 AgP patients and 20 healthy control. Results： MMP-8 and TIMP-1 were positively expressed in the intraepithelial and subcutaneous connective tissue in both groups. MMP-8 expression level and MMP-8/TIMP-1 of AgP group were significantly higher than those of the control group （Z =2.990 and 4. 911 ,P 〈 O. 05 ）, but the difference in TIMP-1 expression between the 2 groups was not significant （ Z = 1. 822, P 〉 0.05 ）. ROC curve analysis showed that AUC（ MMP-8 ） = 0. 752 （ 95% CI = 0. 618 - 0. 885, P = 0. 003 ）, AUC （ MMP-8/TIMP-1 ） = 0.913（95% CI=O. 831 -0. 996,P 〈0. 001 ） ,AUC（TIMP-1 ） = 0. 347（95% CI =0. 194 - 0. 499,P = 0.068）. Conclu- sion： MMP-8 expression level and MMP-8/TIMP-1 in gingival tissue could be helpful for the diagnosis of AgP.

关 键 词：侵袭性牙周炎 基质金属蛋白酶 基质金属蛋白酶抑制因子 

分 类 号：R781.4[医药卫生—口腔医学]