汉黄芩素对胶质瘤U251细胞Surviv inmRNA及Caspase-3 mRNA表达的影响研究  

Effects of wogonin on the expression of Survivin mRNA and Caspase-3 mRNA in human giloma cell line U251

作　　者：黄韶华[1] 梁俊君[1] 董征学[1] 韩庆亮 周文华[1] 

机构地区：[1]山东省安丘市人民医院脑外科,山东安丘262100

出　　处：《中国当代医药》2013年第15期7-8,共2页China Modern Medicine

基　　金：山东省潍坊市科技局科学技术成果(潍科成鉴字[2012]第280号);成果名称:汉黄芩素对胶质瘤细胞U251细胞增殖;凋亡;Sur-vivin及Caspase-3表达的影响

摘　　要：目的探讨汉黄芩素对胶质瘤U251细胞SurvivinmRNA及Caspase-3 mRNA表达的影响。方法 192株人胶质瘤U251细胞株分别用汉黄芩素(96株)、替莫唑胺(TMZ)(48株)处理,留取48株作为空白对照组,半定量RT-PCR法检测Surviv inmRNA与Caspase-3 mRNA表达强度,并在3组之间进行对比分析。结果空白对照组Surviv inmRNA表达强度为(0.773±0.176)μM,显著高于汉黄芩素组的(0.382±0.087)μM及TMZ组的(0.408±0.092)μM(P<0.05);Caspase-3 mRNA表达强度为(0.097±0.043)μM,显著低于汉黄芩素组的(0.454±0.213)μM及TMZ组的(0.432±0.187)μM(P<0.05),汉黄芩组与TMZ组之间比较差异无统计学意义(P>0.05)。结论汉黄芩素可通过下调Surviv inmRNA的表达并上调Caspase-3 mRNA的表达起到有效诱导人胶质瘤U251细胞凋亡的作用。Objective To discuss the effects of wogonin on the expression of Survivin mRNA and Caspase-3 mRNA in human giloma cell line U251. Methods One hundred and ninety-two human giloma cell line U251 were cultured with wogonin （96 strains） and TMZ （48 strains）, and other 48 strains were as blank control group, Survivin mRNA and Cas- pase-3 mRNA expression level were detected by semi-quantitative RT-PCR, and then Survivin mRNA and Caspase- 3 mRNA expression level were contrasted between the three groups. Results Survivin mRNA expression level in the blank control group was （0.773±0.176） μM, significantly higher than （0.382±0.087） μM in the wogonin group and （0.408±0.092） μM in the TMZ group （P 〈 0.05）, and Caspase-3 mRNA expression level was （0.097±0.043） μM, sig- nificantly lower than （0.454±0.213）μM in the wogonin group and （0.432±0.187） μM in the TMZ group （P 〈 0.05）, but there was no significantly difference in Survivin mRNA and Caspase-3 mRNA expression level between wogonin group and TMZ group （P 〉 0.05）. Conclusion Wogonin play a role in inducing apoptosis in glioma U251 cells through re- ducing the expression of Survivin mRNA and raising the expression of Caspase-3 protein expression.

关 键 词：汉黄芩素 胶质瘤 U251 SURVIVIN mRNA CASPASE-3 

分 类 号：R-33[医药卫生]