饲料中添加蝇蛆粉对青鱼非特异性免疫和抗氧化性能的影响  

Effects of Maggot Meal on Non-specific Immunity and Antioxidative Capability of Black Carp (Mylopharyngodon piceus)

作　　者：刘黎[1,2] 叶金云[2] 吴成龙[2] 张易祥[2] 邵仙萍[2] 姜志强[1] 明建华[2] 刘沛[1,2] 

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]湖州师范学院,浙江湖州313000

出　　处：《浙江海洋学院学报（自然科学版）》2013年第2期103-108,118,共7页Journal of Zhejiang Ocean University(Natural Science Edition)

基　　金：国家大宗淡水鱼产业技术体系(CARS-46-21);浙江省重大科技专项重大农业项目(2010C02001);浙江省重点科技创新团队项目(2012R10026-08);湖州市重点科技专项(2010ZD1002);湖州市重点科技创新团队项目(2010KC02)

摘　　要：试验选取420尾青鱼鱼种[初均重(127.42±4.83 g)],随机分成7组,每组3个重复,对照组为基础日粮组,试验组在对照组的基础上添加不同比例的蝇蛆粉(1%、1.8%、2.6%、3.4%、4.2%、5%),试验周期60 d,探讨研究蝇蛆粉对青鱼鱼种非特异性免疫和抗氧化性能的影响。试验结果显示,蝇蛆粉添加量为4.2%时,青鱼鱼种脾脏指数(SBI)最高且较对照组有显著性差异(P<0.05),添加量为5%时青鱼鱼种血清中LZM、C3、C4活性最高且较对照组差异显著(P<0.05),且GOT和GPT活性最低较对照组差异不显著(P>0.05)。饲料中添加4.2%的蝇蛆粉,可显著提高青鱼鱼种血清SOD、GPx、CAT活性(P<0.05),当添加5%的蝇蛆粉时,可显著提高鱼种肝胰脏组织中SOD、GPx、CAT、GR活性显著提高(P<0.05),同时血清和肝胰脏组织中MDA含量显著降低。综上所述,饲料中添加4.2%～5%的蝇蛆粉能显著增强青鱼鱼种的非特异性免疫及抗氧化能力。To study effects of maggot meal （MGM） on black carp （Mylopharyngodon piceus） [initial body weight of （127.42±4.83 g）], a total of 420 black carps were randomly divided into seven groups, the control group was fed with basal diet, the other groups were fed with basal diet supplemented with 1%, 1. 8%; 2.6%, 3.4%, 4.2%, 5% dose of MGM, respectively. After rearing a period of 60 d, the non-specific immunity and antioxidative capability of black carp were investigated. The results showed that the SBI were significantly im- proved by adding 4.2% MGM（P〈0.05） and serum LZM, C3, C4 activities were significantly improved by adding 5% MGM compared with control group（P〈0.05）, but GOT and GPT activities in serum were decreased by adding 5% MGM（P〉0.05）. Black carps fed diet with 4.2% MGM had significant higher activities of SOD, GPx, CAT in serum than control group （P〈0.05）. SOD, GPx and CAT activities in hepatopancreas of black carp were significantly improved by adding 5% MGM （P〈0.05）, but MDA contents in serum and hepatopancreas of black carp were significantly decreased by adding 5% MGM compared with control group （P〈0.05）. The results indicated adding 4.2%-5% MGM could increase the antioxidative capabilitY and non-specific immunity of black carp.

关 键 词：蝇蛆粉 青鱼 非特异性免疫 抗氧化性能 

分 类 号：S963.739[农业科学—水产养殖]